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Structure - Activities

Consortium generated constructs are presented here alphabetically by the name of the protein or portion thereof that they produce. Selecting the construct name will take you to a second table where details of this construct and its availability are listed.

CMC Expression Constructs

Target Amino Acids Portion Produced Construct Name Tag / Cleavable Comments Protocol Contact (Collaborators)
Alpha Actinin 1-892 Alpha actinin - full length pET15b-aActinin His/thrombin soluble protocol Liddington
Alpha Actinin 269-444 Alpha actinin - spectrin repeats pET15b-aActinin-SR His/thrombin soluble Liddington
Alpha Catenin 1-906 Alpha catenin -full length pBS-aCatenin no tag shuttle vector Liddington
Alpha Catenin 277-510 Alpha catenin -domain 3 pET15b-CD3 His/thrombin soluble Liddington
DOCK180 154-256 DOCK180 DHR1 domain pGEX-6P2 ? protocol Liddington
FAK 1-1052 FAK-full length FAK-baculovirus His/non-cleavable soluble expression Liddington (Viori)
FAK 1-1052 FAK-full length pT7-7-FAK no tag protocol Liddington (Viori)
GIT1 1-770 GIT1 -full length GIT1-baculovirus N-ThioHis/enterkinase soluble expression protocol Liddington
GIT1 624-770 GIT1 -paxillin binding domain pGEX4T3-GIT1c GST/thrombin soluble expression protocol Liddington (Horwitz)
Layilin / Talin 207-400 Layilin tail+talin F2+F3 fusion protein pET15b-layilin His/thrombin Liddington
Leupaxin 1-386 Leupaxin -full length pET15b-leupaxin His/HA, His/thrombincleavable, HA not Ginsberg
MEK1 ? Full-length MEK1 pRSETA-MEKK1 His non-cleavable Liddington (Yian)
Paxillin 1-557 Paxillin -full length pET28-paxillin His/thrombin mut in Histag,protein not in frame with start Liddington (Schwarzenbacher)
Paxillin 38-557 Paxillin (N-terminal truncated) pGEX4T2-paxillin 38-557 GST/FLAG, GST-TEVcleavable FLAG not not soluble poor yield Liddington (Vogel)
Plectin 59-293 Plectin - Actin binding domain (ABD) pET15b-plectin6 His/thrombin soluble/great yield protocol Liddington (Pereda)
Plectin 59-293 Plectin -ABD domain mutant pET15b-plectin6ss-mut His/thrombin soluble/great yield
Talin 1-400 Talin Head domain pET15b-His-Talin Head His/thrombin soluble/ok yield protocol Liddington (Pereda)
Talin 196-400 Talin FERM domain (F2F3 portion) pET15b-His-F2F3 His/thrombin soluble/great yield protocol Liddington (Pereda)
Talin / PIPKIg Talin Fragment G (L2094A/I12095A mutant) pET-29+
Talin / PIPKIg 1-2541 Talin-full length, S429D gain-of-function mutant pET30a-talin His/non-cleavable soluble Liddington (Zhang)
Talin / PIPKIg 1-2541 Full length Talin pET30a-talin His/non-cleavable soluble protocol Liddington (Zhang)
Talin / PIPKIg 1-2541 Talin-full length, S446D gain-of-function mutant pET30a-talin His/non-cleavable soluble Liddington (Zhang)
Talin / PIPKIg 1-2541 Talin-full length, S429D/S446D double gain-of-function mutant pET30a-talin His/non-cleavable soluble Liddington (Zhang)
Talin / PIPKIg 1647-2541 Talin C-terminal fragment pET15b-talin 1647-2541 His/thrombin soluble/great yield protocol Liddington (Critchley)
Talin / PIPKIg 1843-1973 Talin VBS2 pET151-DTOPO talin 1843-1973 His/TEV soluble/good yield protocol Liddington (Critchley)
Talin / PIPKIg 196-889 Talin FERM F2F3 + part of rod pET151-DTOPO talin 196-889 protocol
Talin / PIPKIg 1984-2344 Talin VBS3 pGEX-4T-2
Talin / PIPKIg 2071-2135 Talin's actin-binding domain pGEX-4T-2
Talin / PIPKIg 209-400 Talin F2F3 fused to PIPKIgamma tail pET15b-His-F2F3short His/thrombin soluble/great yield Liddington (Pereda)
Talin / PIPKIg 309-405 Talin FERM F3 portion pET15b-His-F3 His/thrombin low yield protocol Liddington (Pereda)
Talin / PIPKIg 309-405 Talin FERM F3 portion pMAL-cgx-F3,4 MBP-6His/FactorXa soluble/ok yield Liddington
Talin / PIPKIg 397-2541 Talin rod pET30b-talin rod N-His Calpain protocol Liddington (Lam)
Talin / PIPKIg 482-636 Talin's Vinculin-binding domain (VBS1; 4-helix bundle) pET15b-talin 482-636 His/thrombin soluble/great yield protocol Liddington (Critchley)
Talin / PIPKIg 482-655 Talin's VBS1 (autoinhibited 5-helix bundle) pET15b-talin 482-655 His/thrombin soluble/great yield protocol Liddington (Critchley)
Talin / PIPKIg 482-789 Talin rod fragment pET15b-talin 482-789 His/thrombin soluble/great yield protocol Liddington (Critchley)
Talin / PIPKIg 482-889 Talin rod fragment pET151-DTOPO talin 482-889 His/TEV soluble/great yield protocol Liddington (Critchley)
Talin / PIPKIg 482-889 Talin 482-889 stabilized fragment quadruple mutant (T775V/T809I/T833I/T867V) pET151-DTOPO talin 482-889TTTT His/TEV soluble/great yield protocol Liddington (Critchley)
Talin / PIPKIg 755-889 Talin's Vinculin-binding domain 2 (VBS2) pET15b-talin 755-889 His/thrombin soluble/great yield protocol Liddington (Critchley)
Tensin 1514-1631 Tensin SH2 domain pET15b-SH2 protocol Hanein (Lin)
Tensin 1514-1631 Tensin SH2+PTB domains pET15b-SH2-PTB protocol Hanein (Lin)
Tensin 1653-1786 Tensin PTB domain pET15b-PTB protocol Hanein (Lin)
Vinculin 1-258 Vinculin Head domain (Y100E mutant) pET15b-VD1 protocol Liddington
Vinculin 1-258 Vinculin -head domain 1 pET15b-VD1 Liddington
Vinculin 879-1066 Vinculin Tail (Y1064E mutant) pET15b-VD1 protocol Liddington (Bakolista)
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