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Structure - Activities

Consortium generated constructs are presented here alphabetically by the name of the protein or portion thereof that they produce. Selecting the construct name will take you to a second table where details of this construct and its availability are listed.

Migration-related protein for which CMC constructs exist

Protein Species Amino Acids Protein or portion produced Construct name
Alpha Actinin human 1-892 Alpha actinin - full length pET15b-aActinin
Alpha Actinin human 269-444 Alpha actinin - spectrin repeats pET15b-aActinin-SR
Alpha Catenin mouse 1-906 Alpha catenin -full length pBS-aCatenin
Alpha Catenin mouse 277-510 Alpha catenin -domain 3 pET15b-CD3
DOCK180 human 154-256 DOCK180 DHR1 domain pGEX-6P2
FAK human 1-1052 FAK-full length pT7-7-FAK
FAK mouse 1-1052 FAK-full length FAK-baculovirus
GIT1 human 1-770 GIT1 -full length GIT1-baculovirus
GIT1 human 624-770 GIT1 -paxillin binding domain pGEX4T3-GIT1c
Layilin / Talin mouse / mouse 207-400 Layilin tail+talin F2+F3 fusion protein pET15b-layilin
Leupaxin human 1-386 Leupaxin -full length pET15b-leupaxin
MEK1 human   Full-length MEK1 pRSETA-MEKK1
Paxillin human 1-557 Paxillin -full length pET28-paxillin
Paxillin human 38-557 Paxillin (N-terminal truncated) pGEX4T2-pacillin 38-557
Plectin human 59-293 Plectin - Actin binding domain (ABD) pET15b-plectin6
Plectin human 59-293 Plectin -ABD domain mutant pET15b-plectin6ss-mut
Talin chicken 1-400 Talin Head domain pET15b-His-Talin Head
Talin chicken 196-400 Talin FERM domain (F2F3 portion) pET15b-His-F2F3
Talin / PIPKIg chicken 209-400 Talin F2F3 fused to PIPKIgamma tail pET15b-His-F2F3short
Talin / PIPKIg chicken 309-405 Talin FERM F3 portion pET15b-His-F3
Talin / PIPKIg chicken 309-405 Talin FERM F3 portion pMAL-cgx-F3,4
Talin / PIPKIg human 397-2541 Talin rod pET30b-talin rod
Talin / PIPKIg human 1-2541 Full length Talin pET30a-talin
Talin / PIPKIg human 196-889 Talin FERM F2F3 + part of rod pET151-DTOPO talin 196-889
Talin / PIPKIg human 1-2541 Talin-full length, S429D gain-of-function mutant pET30a-talin
Talin / PIPKIg human 1-2541 Talin-full length, S446D gain-of-function mutant pET30a-talin
Talin / PIPKIg human 1-2541 Talin-full length, S429D/S446D double gain-of-function mutant pET30a-talin
Talin / PIPKIg mouse 482-636 Talin's Vinculin-binding domain (VBS1; 4-helix bundle) pET15b-talin 482-636
Talin / PIPKIg mouse 482-655 Talin's VBS1 (autoinhibited 5-helix bundle) pET15b-talin 482-655
Talin / PIPKIg mouse 482-789 Talin rod fragment pET15b-talin 482-789
Talin / PIPKIg mouse 482-889 Talin rod fragment pET151-DTOPO talin 482-889
Talin / PIPKIg mouse 482-889 Talin 482-889 stabilized fragment quadruple mutant (T775V/T809I/T833I/T867V) pET151-DTOPO talin 482-889TTTT
Talin / PIPKIg mouse 755-889 Talin's Vinculin-binding domain 2 (VBS2) pET15b-talin 755-889
Talin / PIPKIg mouse 1647-2541 Talin C-terminal fragment pET15b-talin 1647-2541
Talin / PIPKIg mouse 1843-1973 Talin VBS2 pET151-DTOPO talin 1843-1973
Talin / PIPKIg human 1984-2344 Talin VBS3 pGEX-4T-2
Talin / PIPKIg human   Talin Fragment G (L2094A/I12095A mutant) pET-29+
Talin / PIPKIg human 2071-2135 Talin's actin-binding domain pGEX-4T-2
Tensin chicken 1653-1786 Tensin PTB domain pET15b-PTB
Tensin   1514-1631 Tensin SH2 domain pET15b-SH2
Tensin   1514-1631 Tensin SH2+PTB domains pET15b-SH2-PTB
Vinculin chicken 1-258 Vinculin -head domain 1 pET15b-VD1
Vinculin chicken 1-258 Vinculin Head domain (Y100E mutant) pET15b-VD1
Vinculin chicken 879-1066 Vinculin Tail (Y1064E mutant) pET15b-VD1
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Construct details

Construct name Amino Acids Protein or portion produced Tag/cleavable Comments Protocol Contact
pET15b-aActinin 1-892 Alpha actinin - full length His/thrombin soluble protocol Liddington
pET15b-aActinin-SR 269-444 Alpha actinin - spectrin repeats His/thrombin soluble   Liddington
pBS-aCatenin 1-906 Alpha catenin -full length no tag shuttle vector   Liddington
pET15b-CD3 277-510 Alpha catenin -domain 3 His/thrombin soluble   Liddington
pGEX-6P2 154-256 DOCK180 DHR1 domain     protocol Liddington
pT7-7-FAK 1-1052 FAK-full length no tag   protocol Liddington (Viori)
FAK-baculovirus 1-1052 FAK-full length His/non-cleavable soluble expression   Liddington (Viori)
GIT1-baculovirus 1-770 GIT1 -full length N-ThioHis/enterkinase soluble expression protocol Liddington
pGEX4T3-GIT1c 624-770 GIT1 -paxillin binding domain GST/thrombin soluble expression protocol Liddington (Horwitz)
pET15b-layilin 207-400 Layilin tail+talin F2+F3 fusion protein His/thrombin     Liddington
pET15b-leupaxin 1-386 Leupaxin -full length His/HA, His/thrombincleavable, HA not     Ginsberg
pRSETA-MEKK1   Full-length MEK1 His non-cleavable     Liddington (Yian)
pET28-paxillin 1-557 Paxillin -full length His/thrombin mut in Histag,protein not in frame with start   Liddington (Schwarzenbacher)
pGEX4T2-pacillin 38-557 38-557 Paxillin (N-terminal truncated) GST/FLAG, GST-TEVcleavable FLAG not not soluble poor yield   Liddington (Vogel)
pET15b-plectin6 59-293 Plectin - Actin binding domain (ABD) His/thrombin soluble/great yield protocol Liddington (Pereda)
pET15b-plectin6ss-mut 59-293 Plectin -ABD domain mutant His/thrombin soluble/great yield    
pET15b-His-Talin Head 1-400 Talin Head domain His/thrombin soluble/ok yield protocol Liddington (Pereda)
pET15b-His-F2F3 196-400 Talin FERM domain (F2F3 portion) His/thrombin soluble/great yield protocol Liddington (Pereda)
pET15b-His-F2F3short 209-400 Talin F2F3 fused to PIPKIgamma tail His/thrombin soluble/great yield   Liddington (Pereda)
pET15b-His-F3 309-405 Talin FERM F3 portion His/thrombin low yield protocol Liddington (Pereda)
pMAL-cgx-F3,4 309-405 Talin FERM F3 portion MBP-6His/FactorXa soluble/ok yield   Liddington
pET30b-talin rod 397-2541 Talin rod N-His Calpain   protocol Liddington (Lam)
pET30a-talin 1-2541 Full length Talin His/non-cleavable soluble protocol Liddington (Zhang)
pET151-DTOPO talin 196-889 196-889 Talin FERM F2F3 + part of rod     protocol  
pET30a-talin 1-2541 Talin-full length, S429D gain-of-function mutant His/non-cleavable soluble   Liddington (Zhang)
pET30a-talin 1-2541 Talin-full length, S446D gain-of-function mutant His/non-cleavable soluble   Liddington (Zhang)
pET30a-talin 1-2541 Talin-full length, S429D/S446D double gain-of-function mutant His/non-cleavable soluble   Liddington (Zhang)
pET15b-talin 482-636 482-636 Talin's Vinculin-binding domain (VBS1; 4-helix bundle) His/thrombin soluble/great yield protocol Liddington (Critchley)
pET15b-talin 482-655 482-655 Talin's VBS1 (autoinhibited 5-helix bundle) His/thrombin soluble/great yield protocol Liddington (Critchley)
pET15b-talin 482-789 482-789 Talin rod fragment His/thrombin soluble/great yield protocol Liddington (Critchley)
pET151-DTOPO talin 482-889 482-889 Talin rod fragment His/TEV soluble/great yield protocol Liddington (Critchley)
pET151-DTOPO talin 482-889TTTT 482-889 Talin 482-889 stabilized fragment quadruple mutant (T775V/T809I/T833I/T867V) His/TEV soluble/great yield protocol Liddington (Critchley)
pET15b-talin 755-889 755-889 Talin's Vinculin-binding domain 2 (VBS2) His/thrombin soluble/great yield protocol Liddington (Critchley)
pET15b-talin 1647-2541 1647-2541 Talin C-terminal fragment His/thrombin soluble/great yield protocol Liddington (Critchley)
pET151-DTOPO talin 1843-1973 1843-1973 Talin VBS2 His/TEV soluble/good yield protocol Liddington (Critchley)
pGEX-4T-2 1984-2344 Talin VBS3        
pET-29+   Talin Fragment G (L2094A/I12095A mutant)        
pGEX-4T-2 2071-2135 Talin's actin-binding domain        
pET15b-PTB 1653-1786 Tensin PTB domain     protocol Hanein (Lin)
pET15b-SH2 1514-1631 Tensin SH2 domain     protocol Hanein (Lin)
pET15b-SH2-PTB 1514-1631 Tensin SH2+PTB domains     protocol Hanein (Lin)
pET15b-VD1 1-258 Vinculin -head domain 1       Liddington
pET15b-VD1 1-258 Vinculin Head domain (Y100E mutant)     protocol Liddington
pET15b-VD1 879-1066 Vinculin Tail (Y1064E mutant)     protocol Liddington (Bakolista)
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