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Structure - Protocols

Structure Initiative protocols largely describe the expression and purification protocols developed to generate proteins for structure studies.

Software developed for analyzing data are described elsewhere.

Return to the Protocols index.

Differential scanning calorimetry (DSC)

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory doi: 10.1038/nprot.2009.18 Publication date: February 2009

GIT1 (full length) protein purification (Baculovirus)

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory doi: 10.1038/nprot.2009.19 Publication date: February 2009

Production of FAK in baculovirus system

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory doi: 10.1038/nprot.2009.17 Publication date: February 2009

Tensin-PTBc expression and purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory doi: 10.1038/nprot.2009.41 Publication date: February 2009

Tensin-SH2 expression and purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory doi: 10.1038/nprot.2009.42 Publication date: February 2009

Tensin SH2-PTB (ScPc) expression and purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory doi: 10.1038/nprot.2009.20 Publication date: February 2009

Vinculin full length, expression and purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory doi: 10.1038/nprot.2009.45 Publication date: February 2009

Vinculin Head domain 1 (Vd1 and Vd1 Y100E mutant): Expression and purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory doi: 10.1038/nprot.2009.43 Publication date: February 2009

Vinculin Tail (878-1065aa, Wt and Y1064 mutant), expression and purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory doi: 10.1038/nprot.2009.44 Publication date: February 2009

Alpha-actinin-1 expression & purification

Author(s): Liddington and Bankston Lab/Group: Liddington Laboratory Publication date: -

Binding Assay Protocols for Integrin & Talin mutants that block activation in cells

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

DOCK 180 DHR1 domain cloning, expression & NMR

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Electron Microscopy of the Cytoskeleton

Author(s): Tatyana Svitkina and Gary Borisy Lab/Group: Borisy Laboratory Publication date: -

GIT1 (PDB) expression and purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

His-F3-Talin purification &15N/13C labeling

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Isothermal Titration Calorimetry

Author(s): Liddington and Bankston Lab/Group: Liddington Laboratory Publication date: -

Plectin Actin Binding Domain purification & expression

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Preparation of Cy3-Tubulin

Author(s): John Peloquin Lab/Group: Borisy Laboratory Publication date: -

Preparation of Rhodamine-Tubulin

Author(s): John Peloquin Lab/Group: Borisy Laboratory Publication date: -

Talin (1647-2541, C-terminal fragment) expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin (1843-1973) expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin (196-400) F2+3 expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin (196-889 - FERM F2F3 & part of rod) expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin (209-400) F2+3 expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin (482-636 - VBS1; 4-helix bundle) expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin (482-655 -VBS1 autoinhibited 5-helix bundle) expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin (482-789 - rod fragment) expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin (482-889 - rod fragment) expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin (755-889 -VBS2) expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin F3 NMR protocol

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin full length expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin head expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin MBP-F3 expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Talin rod expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -

Vinculin tail expression & purification

Author(s): Robert Liddington and Andrey Bobkov Lab/Group: Liddington Laboratory Publication date: -