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Proteomics - Protocols

Proteomics Initiative protocols describe the proteomic methodologies developed for identifying migration related proteins, including; expression, labeling, purification and mass spectrometry approaches.

Not all the protocols presented here have been integrated into the Nature Protocols Network, in these cases link to the existing file or page are provided.

Software developed for analyzing data are described here.

Return to the Protocols index.

Full Protein Sequence Mapping using Mass Spectrometry

Author(s): Donald Hunt and Erin Jeffery Lab/Group: Hunt Laboratory doi: 10.1038/nprot.2009.50 Publication date: February 2009

Identification of Utilized Phosphorylation Sites in Tagged Proteins

Author(s): Nicholas Sherman and Jay Fox Lab/Group: Fox Laboratory doi: 10.1038/nprot.2008.202 Publication date: September 2008

Method for Full Protein Sequence Mapping: LC-MS analysis

Author(s): Erin D. Jeffery Lab/Group: Hunt Laboratory doi: 10.1038/nprot.2007.34 Publication date: January 2007

Method for Full Protein Sequence Mapping: LC-MS sample preparation

Author(s): Erin D. Jeffery Lab/Group: Hunt Laboratory doi: 10.1038/nprot.2007.33 Publication date: January 2007

SILAC Protocols

Author(s): Larry Goldfinger and Mark Ginsberg Lab/Group: Ginsberg Laboratory Publication date: February 2006

Desalting and Ion Exchange Procedures

Author(s): Horwitz Lab/Group: Horwitz Laboratory Publication date: -

IMAC Enrichment of Phosphopeptides (Step-by-Step IMAC Protocol)

Author(s): Donald Hunt and Erin Jeffery Lab/Group: Hunt Laboratory Publication date: -

IP procedure for MS

Author(s): Horwitz Lab/Group: Horwitz Laboratory Publication date: -

Protein Solubilization

Author(s): Parsons Lab/Group: Parsons Laboratory Publication date: -

Silver Stain Methods

Author(s): Parsons Lab/Group: Parsons Laboratory Publication date: -

Standard Digestion Protocols

Author(s): Parsons Lab/Group: Parsons Laboratory Publication date: -

Stress Fiber Isolation

Author(s): Keith Burridge Lab/Group: Burridge Laboratory Publication date: -

TAP-Ras Protocols

Author(s): Larry Goldfinger and Mark Ginsberg Lab/Group: Ginsberg Laboratory Publication date: -

ZipTip Purification of Peptides and Proteins

Author(s): Parsons Lab/Group: Parsons Laboratory Publication date: -