Proteomics - Protocols
Proteomics Initiative protocols describe the proteomic methodologies developed for identifying migration related proteins, including; expression, labeling, purification and mass spectrometry approaches.
Not all the protocols presented here have been integrated into the Nature Protocols Network, in these cases link to the existing file or page are provided.
Software developed for analyzing data are described here.
Return to the Protocols index.
Full Protein Sequence Mapping using Mass Spectrometry
Lab/Group: Hunt Laboratory doi: 10.1038/nprot.2009.50 Publication date: February 2009
Identification of Utilized Phosphorylation Sites in Tagged Proteins
Lab/Group: Fox Laboratory doi: 10.1038/nprot.2008.202 Publication date: September 2008
Method for Full Protein Sequence Mapping: LC-MS analysis
Lab/Group: Hunt Laboratory doi: 10.1038/nprot.2007.34 Publication date: January 2007
Method for Full Protein Sequence Mapping: LC-MS sample preparation
Lab/Group: Hunt Laboratory doi: 10.1038/nprot.2007.33 Publication date: January 2007
SILAC Protocols
Lab/Group: Ginsberg Laboratory Publication date: February 2006
Desalting and Ion Exchange Procedures
Lab/Group: Horwitz Laboratory Publication date: -
IMAC Enrichment of Phosphopeptides (Step-by-Step IMAC Protocol)
Lab/Group: Hunt Laboratory Publication date: -
IP procedure for MS
Lab/Group: Horwitz Laboratory Publication date: -
Protein Solubilization
Lab/Group: Parsons Laboratory Publication date: -
Silver Stain Methods
Lab/Group: Parsons Laboratory Publication date: -
Standard Digestion Protocols
Lab/Group: Parsons Laboratory Publication date: -
Stress Fiber Isolation
Lab/Group: Burridge Laboratory Publication date: -
TAP-Ras Protocols
Lab/Group: Ginsberg Laboratory Publication date: -
ZipTip Purification of Peptides and Proteins
Lab/Group: Parsons Laboratory Publication date: -
