Experimental Methods

ZYXIN phosphorylation site summary


Peptides - zyxin_c18_inh2.aln

                                                                                                               
zyxin      mvskGEELFTGVVPILVELDGDVNGHKfsvsgegegdatygkltlkficttgklpvpwptlvttltygvqcfsrYPDHMKqhdffkSAMPEGYVQERTIF
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
               GEELFTGVVPILVELDGDVNGHK                                                                      TIF                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                                 SAMPEGYVQER                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             

                                                                                                               
zyxin      FKDDGNYKTRAEVKFEGDTLVNRielkGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKvnfkirHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                   
           FKDDGNYK                   GIDFKEDGNILGHK                                                                                                                                                                                                                                                                                                                                                                       
                         FEGDTLVNR                  LEYNYNSHNVYIMADK                                                                                                                                                                                                                                                                                                                                                       

                                                        1       10        20        30        40        50     
zyxin      YLSTQSALSKdpnekrDHMVLLEFVTAAGITLGMDELYKipefrAMAAPRPPPAISVSVSAPAFYAPQKKFAPVVAPKPKVNPFRPGDSEPPVAAGAQRa
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                       AMAAPRPPPAISVSVSAPAFYAPQKK                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                                                                           VNPFRPGDSEPPVAAGAQR                                                                                                                                                                                                                                                                                                                                                                                                       

              60        70        80        90       100       110       120       130       140       150     
zyxin      qmgrvgeipppppedfplppppligegddsegalggafppppppmieepfppapleedIFPSPPPPLEEEGGPEAPTQLPPQPREKVCSIDLEIDSLSSL
All Expts                                                                                          *           
C18_INH2                                                                                           *                                                                                                                                                                                                                             
                                                                                               EKVCSIDLEIDSLSSL                                                                                                                                                                                                                  
                                                                                                                                                                                                                                                                                                                                 

             160       170       180       190       200       210       220       230       240       250     
zyxin      LDDMTKNDPFKarvssgyvpppvatpfvpkpstkpapggtaplppwKTPSSSQPPPQPQAKPQVQLHVQPQAKPHVQPQPVSSANTQPRGPLSQAPTPAP
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
           LDDMTK                        PSTKPAPGGTAPLPPWK                                          GPLSQAPTPAP                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                        VSSGYVPPPVATPFVPK                               PQVQLHVQPQAKPHVQPQPVSSANTQPR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         

             260       270       280       290       300       310       320       330       340       350     
zyxin      KFAPVAPKFTPVVSKFSPGAPSGPGPQPNQKMVPPDAPSSVSTGSPQPPSFTYAQQKEKPLVQEKQHPQPPPAQNQNQVRSPGGPGPLTLKEVEELEQLT
All Expts                  *                       [   *                                   *                   
C18_INH2                                                                                   *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
           K              FSPGAPSGPGPQPNQK                                                 SPGGPGPLTLKEVEELEQLT                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
            FAPVAPK                       MVPPDAPSSVSTGSPQPPSFTYAQQK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             

             360       370       380       390       400       410       420       430       440       450     
zyxin      QQLMQDMEHPQRQSVAVNESCGKCNQPLARaqpavrALGQLFHITCFTCHQCQQQLQGQQFYSLEGAPYCEGCYTDTLEKCNTCGQPITDRmlratgkAY
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
           QQLMQDMEHPQR                        ALGQLFHITCFTCHQCQQQLQGQQFYSLEGAPYCEGCYTDTLEK                  AY                                                                                                                                                                                                                                                                                                                                                                                                                                    
                       QSVAVNESCGKCNQPLAR                                                  CNTCGQPITDR                                                                                                                                                                                                                                                                                                                                                                                                                                             

             460       470       480       490       500       510       520       530       540       550     
zyxin      HPQCFTCVVCACPLEGTSFIVDQANQPHCVPDYHKqyaprCSVCSEPIMPEPGRDETVRVVALDKNFHMKcykCEDCGKPLSIEADDNGCFPLDGHVLCR
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                   
           HPQCFTCVVCACPLEGTSFIVDQANQPHCVPDYHK                        VVALDKNFHMK                                                                                                                                                                                                                                                                                                                                          
                                                   CSVCSEPIMPEPGRDETVR              CEDCGKPLSIEADDNGCFPLDGHVLCR                                                                                                                                                                                                                                                                                                            

             560       570       580       590       600       610       620       630       640       650     
zyxin      kchsaraqteqklimekrrwkKNFIAVSAANRfkKISSSGALDYDIPTTASENLYFQGELKTAALAQHDEAVDNKFNKeqqnafyeilhlpnlneeqrna
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                KNFIAVSAANR                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                             KISSSGALDYDIPTTASENLYFQGELK                                                                                                                                                                                                                                                                                                                                                                                                                                             

             660       670       680       690       700       710       720       730       740       750  
zyxin      fiqslkddpsqsanllaeakklndaqapkvdnkFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLLAEAKKLNGAQAPKvdansagkst
All Expts                                                                                                   
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                
                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                                                                                                                                                        


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB
IMAC_INHIB
TIO_INHIB
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.