Experimental Methods

WASP phosphorylation site summary


Peptides - wasp_c18_inhib1.aln

                        1       10        20        30        40        50        60        70        80       
wasp       DYKDDDDKegvrtmSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFirL
All Expts                                                                                                      
C18_INHIB1                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                                                                                                                                                                                                                                                                                                                                         

            90       100       110       120       130       140       150       160       170       180       
wasp       YGLQAGRLLWEQELYSQLVYstptpffhtfagddcqaglnfadedeaqafralvqekiqkrnqrqsgdrrQLPPPPTPANEERRGGLPPLPLHPGGDQGG
All Expts                                                                              *                       
C18_INHIB1                                                                             *                                                                                                                                                                                                                                                                                                                                   
                                                                                 QLPPPPTPANEER                                                                                                                                                                                                                                                                                                                             
                                                                                              RGGLPPLPLHPGGDQGG                                                                                                                                                                                                                                                                                                            

           190       200       210       220       230       240       250       260       270       280       
wasp       PPVGPLSLGLATVDIQNPDITSSRYRGLPAPGPSPADKKRSGKKKISKADIGAPSGFKHVSHVGWDPQNGFDVNNLDPDLRSLFSRAGISEAQLTDAETS
All Expts        *                          *                          *                                       
C18_INHIB1       *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
           PPVGPLSLGLATVDIQNPDITSSR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          

           290       300       310       320       330       340       350       360       370       380       
wasp       KLIYDFIEDQGGLEAVRQEMRRQEPLPPPPPPSRGGNQLPRPPIVGGNKGRSGPLPPVPLGIAPPPPTPRgppppgrGGPPPPPPPATGRSGPLPPPPPG
All Expts     *                                               *                                                
C18_INHIB1                                                                                                                                                                                                                                                                                         
                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                   

           390       400       410       420       430       440       450       460       470       480       
wasp       AGGPPMPPPPPPPPPPPSSGNGPAPPPLPPALVPAGGLAPGGGRGALLDQIRqgiqlNKTPGAPESSALQPPPQSSEGLVGALMHVMQKrsrAIHSSDEG
All Expts                                                                            ??                   **   
C18_INHIB1                                                                                                **                                                                                                                                                                                                                                                                                                               
                                                                                                       AIHSSDEG                                                                                                                                                                                                                                                                                                            
                                                                                                                                                                                                                                                                                                                                                                                                                           

           490       500  
wasp       EDQAGDEDEDDEWDD
All Expts                 
C18_INHIB1                
           EDQAGDEDEDDEWDD
                          


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB
IMAC_INHIB
TIO_INHIB
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.