Experimental Methods

VAV1 phosphorylation site summary


Peptides - vav1_c18_inh2.aln

                        1       10        20        30        40        50        60        70        80       
vav1       DYKDDDDKKEFGTMELWRQCTHWLIQCRvlppshrVTWDGAQVCELAQALRDGVLLCQLLNNLLPHAINLREVNLRPQMSQFLCLKnirtflsTCCEKFG
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                             

            90       100       110       120       130       140       150       160       170       180       
vav1       LKRSELFEAFDLFDVQDFGKVIYTLSALSWTPIAQNRGIMPFPTEEESVGDEDIYSGLSDQIDDTVEEDEDLYDCVENEEAEGDEIYEDLMRSEPVSMPP
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               

           190       200       210       220       230       240       250       260       270       280       
vav1       KmteydkrccclreiqqteekYTDTLGSIQQHFLKPLQRFLKPQDIEIIFINIEDLLRVHTHFLKEMKEALGTPGAANLYQVFIKykerflvygrYCSQV
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

           290       300       310       320       330       340       350       360       370       380       
vav1       ESASKhldrVAAAREDVQMKLEECSQRanngrFTLRDLLMVPMQRVLKYHLLLQELVKHTQEAMEKENLRLALDAMRDLAQCVNEVKRdnetlrQITNFQ
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                               

           390       400       410       420       430       440       450       460       470       480       
vav1       LSIENLDQSLAHYGRPKIDGELKITSVERrskMDRYAFLLDKALLICKRRGDSYDLKDFVNLHSFQVRddssgdrdnkKWSHMFLLIEDQGAQGYELFFK
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

           490       500       510       520       530       540       550       560       570       580       
vav1       trelkkkWMEQFEMAISNIYPENATANGHDFQMFSFEETTSCKACQMLLRGTFYQGYRchrcrasahkeclgrvppcgrHGQDFPGTMKKdklhrraqdk
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     

           590       600       610       620       630       640       650       660       670       680       
vav1       kRNELGLPKMEVFQEYYGLPPPPGAIGPFLRLNPGDIVELTKAEAEQNWWEGRNTSTNEIGWFPCNRVKPYVHGPPQDLSVHLWYAGPMERAGAESILAN
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                           

           690       700       710       720       730       740       750       760       770       780       
vav1       RSDGTFLVRQRVKDAAEFAISIKYNVEVKHIKIMTAEGLYRITEKkafrGLTELVEFYQQNSLKDCFKSLDTTLQFPFKEPEKRTISRPAVGSTKYFGTA
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         

           790       800       810       820       830       840     
vav1       KARYDFCARDRSELSLKEGDIIKilnkKGQQGWWRGEIYGRVGWFPANYVEEDYSEYC
All Expts                                                          * 
C18_INH2                                                           *                                                                                                                                                                                                                                                 
                                                    VGWFPANYVEEDYSEYC                                                                                                                                                                                                                                                
                                                                                                                                                                                                                                                                                                                     


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB
IMAC_INHIB
TIO_INHIB
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.