Experimental Methods

Talin phosphorylation site summary


Peptides - talin_c18_inhib.aln

                   10        20        30        40        50        60        70        80        90       100
TLN1_HUMAN mVALSLKISIGNVVKTMQFEPSTMVYDACRiirERIPEAPAGPPSDFGLFLSDDDPKKGIWLEAGKALDYYMLRNGDTMEyrkkqrplkIRMLDGTVKTI
All Expts      *                    *                                           *       *                 *    
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                  110       120       130       140       150       160       170       180       190       200
TLN1_HUMAN MVDDSKTVTDMLMTICARIGITNHDEyslvrelmEEKKEEITGTLRKdkTLLRDEKkmeKLKQKLHTDDELNWLDHGRTLREQGVEEhetlllrrkFFYS
All Expts               *            **               *     *                                       *          
C18_INHIB                                             *     *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                                KEEITGTLR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                          DKTLLRDEK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              

                  210       220       230       240       250       260       270       280       290       300
TLN1_HUMAN DQNVDSRDPVQLNLLYVQARDDILNGSHPVSFDKACEFAGFQCQIQFGPHNEQKhkAGFLDLKDFLPKEYVKqkgerkifqahkNCGQMSEIEAKvryvk
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                 

                  310       320       330       340       350       360       370       380       390       400
TLN1_HUMAN larslkTYGVSFFLVKEkmkgkNKLVPRLLGITKEcvmrvdekTKEVIQEWNLTNIKRWAASPKsftldFGDYQDGYYSVQTTEGEQIAQLIAGYIDIIL
All Expts            *                                                                                         
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             

                  410       420       430       440       450       460       470       480       490       500
TLN1_HUMAN KKkkSKDHFGLEGDEESTMLEDSVSPKKSTVLQQQYNRVGKvehgsvalpaimrSGASGPENFQVGSMPPAQQQITSGQMHRGHMPPLTSAQQALTGTIN
All Expts                          *   []     *         *        [  ]        *                                 
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           

                  510       520       530       540       550       560       570       580       590       600
TLN1_HUMAN SSMQAVQAAQATLDDFDTLPPLGQDAASKawrknkmdeskHEIHSQVDAITAGTASVVNLTAGDPAETDYTAVGCAVTTISSNLTEMSRgvkLLAALLED
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

                  610       620       630       640       650       660       670       680       690       700
TLN1_HUMAN EGGSGRPLLQAAKGLAGAVSELLRSAQPASAEPRQNLLQAAGNVGQASGELLQQIGESDTDPHFQDALMQLAKAVASAAAALVLKAKSVAQRTEDSGLQT
All Expts                                                                              *                       
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

                  710       720       730       740       750       760       770       780       790       800
TLN1_HUMAN QVIAAATQCALSTSQLVACTKVVAPTISSPVCQEQLVEAGRLVAKAVEGCVSASQAATEDGQLLRGVGAAATAVTQALNELLQHVKAHATGAGPAGRYDQ
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                  810       820       830       840       850       860       870       880       890       900
TLN1_HUMAN ATDTILTVTENIFSSMGDAGEmvgqarILAQATSDLVNAIKADAEGESDLENSRkLLSAAKILADATAKmveaakgaaahpdseeqQQRLREAAEGLRMA
All Expts                *                                                                                     
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     

                  910       920       930       940       950       960       970       980       990      1000
TLN1_HUMAN TNAAAQNAIKKKLVQRlehaakQAAASATQTIAAAQHAASTPKASAGPQPLLVQSCKAVAEQIPLLVQGVRGSQAQPDSPSAQLALIAASQSFLQPGGKm
All Expts                                          *                                     * *                   
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     

                 1010      1020      1030      1040      1050      1060      1070      1080      1090      1100
TLN1_HUMAN vaaakASVPTIQDQASAMQLSQCAKNLGTALAELRTAAQKAQEACGPLEMDSALSVVQNLEKDLQEVKAAARDGKLKPLPGETMEKCTQDLGNSTKavss
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                 1110      1120      1130      1140      1150      1160      1170      1180      1190      1200
TLN1_HUMAN aiaqllgeVAQGNENYAGIAARDVAGGLRSLAQAARGVAALTSDPAVQAIVLDTASDVLDKASSLIEEAKKAAGHPGDPESQQRLAQVAKAVTQALNRCV
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                           

                 1210      1220      1230      1240      1250      1260      1270      1280      1290      1300
TLN1_HUMAN SCLPGQRDVDNALRAVGDASKrllsDSLPPSTGTFQEAQSRLNEAAAGLNQAATELVQASRGTPQdlarasgrFGQDFSTFLEAGVEMAGQAPSQEDRaq
All Expts  *                                                                                                   
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                 1310      1320      1330      1340      1350      1360      1370      1380      1390      1400
TLN1_HUMAN vvsnlkgismsssklllaakALSTDPAAPNLKSQLAAAARAVTDSINQLITMCTQQAPGQKECDNALRELETVRELLENPVQPINDMSYFGCLDSVMENS
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             

                 1410      1420      1430      1440      1450      1460      1470      1480      1490      1500
TLN1_HUMAN KVLGEAMTGISQNAKNGNLPEFGDAISTASKalcgfteaaaqaaylvgvsDPNSQAGQQGLVEPTQFARANQAIQMACQSLGEPGCTQAQVLSAATIVAK
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                 1510      1520      1530      1540      1550      1560      1570      1580      1590      1600
TLN1_HUMAN HTSALCNSCRLASARTTNPTAKrqfvqsakEVANSTANLVKtikaldgpfteENRAQCRAATAPLLEavDNLSAFASNPEFSSIPAQISPEGRAAMEPIV
All Expts         *                                                                                            
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           

                 1610      1620      1630      1640      1650      1660      1670      1680      1690      1700
TLN1_HUMAN ISAQtmleSAGGLIQTARALAVNPRDPPSWSVLAGHSRTVSDSIKKLITSMRDKapgqleCETAIAALNSCLRDLDQASLAAVSQQLAPREGISQEALHT
All Expts                                          *                                          *                
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           

                 1710      1720      1730      1740      1750      1760      1770      1780      1790      1800
TLN1_HUMAN EMLTAVQEishlieplahaaraeasqlghkVSQMAQYFEPLTLAAVGAASKTLSHPQQMALLDQTKTLAESALQLLYTAKEAGGNPKQAAHTQEALEEav
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                 1810      1820      1830      1840      1850      1860      1870      1880      1890      1900
TLN1_HUMAN qmmteAVEDLTTTLNEAASAAGVVGGMVDSITQAINQLDEGPMGEPEGSFVDYQTTMVRTAKAIAVTVQEMVTKSNTSPEELGPLANQLTSDYGRLASEA
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     

                 1910      1920      1930      1940      1950      1960      1970      1980      1990      2000
TLN1_HUMAN KPAAVAAENEEIGSHIKhrVQELGHGCAALVTKAGALQCSPSDAYTKKELIEcarrvsekVSHVLAALQAGNRGTQACITAASAVSGIIAdlDTTIMFAT
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                 2010      2020      2030      2040      2050      2060      2070      2080      2090      2100
TLN1_HUMAN AGTLNREGTETFADHREGILKtakVLVEDTKVLVQNAAGSQEKLAQAAQSSVATITRLADVVKLGAASLGAEDPETQVVLINAVKDVAKALGDLISATKa
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                 2110      2120      2130      2140      2150      2160      2170      2180      2190      2200
TLN1_HUMAN aagkvgddpavwqLKNSAKVMVTNVTSLLKTVKAVEDEATKGTRALEATTEHIRQELAVFCSPEPPAKtstpedfirmtkGITMATAKAVAAGNSCRQED
All Expts                            *                                                                         
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             

                 2210      2220      2230      2240      2250      2260      2270      2280      2290      2300
TLN1_HUMAN VIATANLSRRAIADMLRACKEAAYHPEVAPDVRlralhygrECANGYLELLDHVLLTLQKPSPELKqqltghskRVAGSVTELIQAAEAMKGTEWVDPED
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

                 2310      2320      2330      2340      2350      2360      2370      2380      2390      2400
TLN1_HUMAN PTVIAENELLGAAAAIEAAAKKLEQLKPRAKPKEADESLNFEEQILEAAKSIAAATSALVKAASAAQRELVAQGKVGAIPANALDDGQWSQGLISAARMV
All Expts                                       *                                                              
C18_INHIB                                                                                                                                                                                                                                                                                                                                                      
                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                               

                 2410      2420      2430      2440      2450      2460      2470      2480      2490      2500
TLN1_HUMAN AAATNNLCEAANAAVQGHASQEKlissakQVAASTAQLLVACKVKADQDSEAMKRlqaagnavkRASDNLVKAAQKAAAFEeqenETVVVKEKMVGGIAQ
All Expts                                                                                                      
C18_INHIB                                                                                                                                                                                                                                                                                                                                                                                    
                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                             

                 2510      2520      2530      2540 
TLN1_HUMAN IIAAQEEMLRkereleearkKLAQIRQQQYKFLPSELRDEH
All Expts                               *    *      
C18_INHIB                                                                                                       
                                                                                                                
                                                                                                                


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB
IMAC_INHIB
TIO_INHIB
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.


Experimental Methods will be available shortly. Typical experimental methods are described for Paxillin.