Experimental Methods

RHOGEF phosphorylation site summary


Peptides - rho_tio_inhib3.aln

                        1       10        20        30        40        50        60        70        80       
rhogef     dyKDDDDKEGVRTMEDFARGAASPGPSRPGLVPVSIIGAEDEDFENELEtnseeqnsqfqsleqvkrRPAHLMALLQHVALQFEPGPLLCCLHADMLGSL
All Expts                        *                                                                             
TIO_INHIB3                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                           

            90       100       110       120       130       140       150       160       170       180       
rhogef     GPKEAKKAFLDFYHSFLEKTAVLRVPVPPNVAFELDRTRADLISEDVQRRFVQEVVQSQQVAVGRQLEdfrskRLMGMTPWEQELAQLEAWVGRdrasyE
All Expts                                                                                                      
TIO_INHIB3                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                           

           190       200       210       220       230       240       250       260       270       280       
rhogef     ARERHVAERLLMHLEEMQHTISTDEEKSAAVVNAIGLYMRHLGVRTKSGDKksgrnffrkkvmgnrrsddppkTKKGLSSILDAARWNRGEPQVPDFRHL
All Expts                                                                                **                    
TIO_INHIB3                                                                               **                                                                                                                                                                                                                                                                                                                                                                                            
                                                                                      KGLSSILDAAR                                                                                                                                                                                                                                                                                                                                                                                      
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

           290       300       310       320       330       340       350       360       370       380       
rhogef     KAEVDAEKPGATDRKGGVGMPSRDRNIGAPGQDTPGVSLHPLSLDSPDREPGADAPLELGDSSPQGPMSLESLAPPESTDEGAETESPEPGDEGEPGRSG
All Expts                                   *        *  *                *              *      * *           * 
TIO_INHIB3                                  *           *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                    NIGAPGQDTPGVSLHPLSLDSPDR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     

           390       400       410       420       430       440       450       460       470       480       
rhogef     LELEPEEPPGWRELVPPDTLHSLPKSQVKRQEVISELLVTEAAHVRMLrVLHDLFFQPMAECLFFPLEELQNIFPSLDELIEVHSLFLDRLMkRRQESGY
All Expts                                                                                                      
TIO_INHIB3                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                             

           490       500       510       520       530       540       550       560       570       580       
rhogef     LIEEIGDVLLARFDGAEGSWFQKissrFCSRQSFALEQLKAKQRKDPRFCAFVQEAESRPRcrRLQLKDMIPTEMQRLTKYPLLLQSIGQNTEEPTEREK
All Expts                                                                                                      
TIO_INHIB3                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

           590       600       610       620       630       640       650       660       670       680       
rhogef     VELAAECCREILHHVNQAVRDMEDLLRLKDYqrRLDLSHLRQSSDPMLSEFKNLDITKKKLVHEGPLTWRVTKDKAVEVHVLLLDDLLLLLQRQDERLLL
All Expts                                             *                                                        
TIO_INHIB3                                            *                                                                                                                                                                                                                                                                                                        
                                                    QSSDPMLSEFK                                                                                                                                                                                                                                                                                                
                                                                                                                                                                                                                                                                                                                                                               

           690       700       710       720       730       740       750       760       770       780       
rhogef     KSHSRtlTPTPDGKTMLRPVLRlTSAMTREVATDHKAFYVLFTWDQEAQIYELVAQTVSERKNWCALITETAGSLKVPAPASRPKprprpsstrEPLLSS
All Expts         *                                                                                          * 
TIO_INHIB3                                                                                                   *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                                         EPLLSS                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                

           790       800       810       820       830       840       850       860       870       880       
rhogef     SENGNGGRetspaDARTERILSDLLPFCRPGPEGQLAATALRKVLSLKQLLFPAEEDNGAGPPRDGDGVPGGGPLSPARTQEIQENLLSLEETMKQLEEL
All Expts                                                                             *                        
TIO_INHIB3                                                                            *                                                                                                                                                                                                                                                                                                                                                                  
                                                           QLLFPAEEDNGAGPPRDGDGVPGGGPLSPAR                                                                                                                                                                                                                                                                                                                                                               
           SEDGNGGRETSPADAR                                                                                                                                                                                                                                                                                                                                                                                                                              

           890       900       910  
rhogef     EEEFCRLRPLLSQLGGNSVPQPGCT
All Expts                           
TIO_INHIB3                                                                                                                    
                                                                                                                              
                                                                                                                              


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB
IMAC_INHIB
TIO_INHIB
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.