| Phosphoproteomics |
Phosphorylation of FLAG-PAXILLIN
CMCKB: FLAG-Paxillin (PXN, chicken)
Sites: S-2, T29, Y31, Y40, Y76, S83, S85, Y88, S98, S106, S108, S112, Y118, S119, S126, S130, S137, S143, S164, S171, S173, Y182, T199, S228, S231, S245, S259, S262, S273, S275, S290, T295, S308, S361, Y377, S382, Y436, S501, S504, T540
| C18 (+) inhibitors | Alignment / Methods |
| IMAC (+) inhibitors | Alignment / Methods |
| Site | Position | Peptide | ||
|---|---|---|---|---|
| (+) Inhib | ||||
| -2S | -8 - 20 | LGTELGSGTMDDLDALLADLESTTSHISK | ||
| 29T | 21 - 31 | RPVFLTEETPY | ||
| 31Y | 21 - 37 | RPVFLTEETPYSYPTGN | # | |
| 31Y / 40Y | 28 - 42 | ETPYSYPTGNHTYQE | ||
| 76Y / 88Y | 76 - 93 | YGHQQPQSQSPIYSSSAK | ||
| 83S / 85S / 88Y | 76 - 93 | YGHQQPQSQSPIYSSSAK | ||
| 85S | 76 - 93 | YGHQQPQSQSPIYSSSAK | # | |
| 85S / 88Y | 76 - 93 | YGHQQPQSQSPIYSSSAK | ||
| 85S / 89S-91S | 76 - 93 | YGHQQPQSQSPIY[SSS]AK | ||
| 88Y | 76 - 93 | YGHQQPQSQSPIYSSSAK | # | |
| 94S-96S | 94 - 101 | [SSS]ASVPR | ||
| 98S | 94 - 101 | SSSASVPR | # | |
| 98S / 106S | 94 - 110 | SSSASVPRDGLSSPSPR | ||
| 105S-106S | 102 - 110 | DGL[SS]PSPR | ||
| 106S | 102 - 110 | DGLSSPSPR | # | |
| 106S / 108S | 102 - 110 | DGLSSPSPR | ||
| 108S | 102 - 110 | DGLSSPSPR | # | |
| 112S | 111 - 123 | ASEEEHVYSFPNK | ||
| 118Y | 111 - 123 | ASEEEHVYSFPNK | # | |
| 118Y / 119S | 111 - 123 | ASEEEHVYSFPNK | ||
| 126S | 124 - 147 | QKSAEPSPTMTSTSLGSNLSELDR | # | |
| 126S / 130S | 124 - 147 | QKSAEPSPTMTSTSLGSNLSELDR | ||
| 130S | 126 - 147 | SAEPSPTMTSTSLGSNLSELDR | # | |
| 137S | 126 - 147 | SAEPSPTMTSTSLGSNLSELDR | ||
| 143S | 140 - 147 | SNLSELDR | ||
| 164S | 148 - 170 | LLLELNAVQHNPPSGFSADEVSR | ||
| 171S | 171 - 194 | SPSLPNVTGPHYVIPESSSSAGGK | # | |
| 173S | 171 - 194 | SPSLPNVTGPHYVIPESSSSAGGK | # | |
| 173S / 182Y | 171 - 194 | SPSLPNVTGPHYVIPESSSSAGGK | ||
| 182Y | 171 - 194 | SPSLPNVTGPHYVIPESSSSAGGK | # | |
| 187S-190S | 171 - 194 | SPSLPNVTGPHYVIPE[SSSS]AGGK | ||
| 199T | 195 - 204 | AAPPTKEKPK | ||
| 217S-220S / 227S-228S / 231S / 244S-245S | 210 - 248 | GIEDVRP[SVES]LLDELE[SS]VPSPVPAITVSQGEV[SS]PQR | ||
| 227S-228S | 210 - 248 | GIEDVRPSVESLLDELE[SS]VPSPVPAITVSQGEVSSPQR | ||
| 228S / 231S / 245S | 210 - 248 | GIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQR | ||
| 245S | 210 - 248 | GIEDVRPSVESLLDELESSVPSPVPAITVSQGEVSSPQR | # | |
| 259S | 258 - 265 | ISASSATR | ||
| 262S | 258 - 265 | ISASSATR | # | |
| 273S | 266 - 278 | ELDELMASLSDFK | ||
| 275S | 266 - 278 | ELDELMASLSDFK | ||
| 289S-290S | 285 - 315 | AGGS[SS]PPSTTPKPGSQLDTMLGSLQSDLNK | ||
| 290S | 285 - 315 | AGGSSSPPSTTPKPGSQLDTMLGSLQSDLNK | # | |
| 290S / 295T | 285 - 315 | AGGSSSPPSTTPKPGSQLDTMLGSLQSDLNK | ||
| 308S | 298 - 315 | PGSQLDTMLGSLQSDLNK | ||
| 361S | 344 - 362 | TWHPEHFVCTHCQEEIGSR | ||
| 361S | 344 - 367 | TWHPEHFVCTHCQEEIGSRNFFER | ||
| 377Y | 376 - 384 | YYHNLFSPR | ||
| 382S | 376 - 384 | YYHNLFSPR | ||
| 436Y | 434 - 443 | KDYFDMFAPK | ||
| 501S | 499 - 513 | RGSLCSGCQKPITGR | # | |
| 501S / 504S | 499 - 513 | RGSLCSGCQKPITGR | ||
| 540T | 535 - 556 | QLNKGTFKEQNYKPYCQNCFLK |
*Peptides detected only by
enrichment with
immobilized metal-affinity chromatography (IMAC).
# Peptides which do not require enrichment by IMAC for detection.