| Phosphoproteomics |
Phosphorylation of PAK1
| C18 inhib. | Alignment / Methods |
| IMAC inhib. | Alignment / Methods |
| Site | Position | Peptide | ||
|---|---|---|---|---|
| (+) Inhib | ||||
| -11S--10T | -17 - -6 | LGTELG[ST]SNGR | ||
| 2S | 1 - 18 | MSNNGLDIQDKPPAPPMR | ++++ | |
| 21S | 19 - 29 | NTSTMIGAGSK | ||
| 57S | 55 - 66 | YRSILPGDKTNK | ||
| 131Y | 119 - 134 | KNPQAVLDVLEFYNSK | ++ | |
| 131Y | 120 - 134 | NPQAVLDVLEFYNSK | ++++ | |
| 142Y | 142 - 148 | YMSFTDK | +++ | |
| 153Y | 149 - 162 | SAEDYNSSNALNVK | +++ | |
| 153Y / 155S-156S | 149 - 162 | SAEDYN[SS]NALNVK | ||
| 155S-156S | 149 - 162 | SAEDYN[SS]NALNVK | ||
| 174S | 163 - 193 | AVSETPAVPPVSEDEDDDDDDATPPPVIAPR | +++ | |
| 174S / 185T | 163 - 198 | AVSETPAVPPVSEDEDDDDDDATPPPVIAPRPEHTK | +++ | |
| 212T | 204 - 215 | SVIEPLPVTPTR | ++++ | |
| 285Y | 276 - 299 | IGQGASGTVYTAMDVATGQEVAIK | + | |
| 429Y | 422 - 438 | STMVGTPYWMAPEVVTR | ++ | |
| 464Y | 454 - 470 | AIEMIEGEPPYLNENPL |
*Peptides detected only by
enrichment with
immobilized metal-affinity chromatography (IMAC).
# Peptides which do not require enrichment by IMAC for detection.