Experimental Methods

NWASP phosphorylation site summary


Peptides - nwasp_c18_inhibb.aln

                   10        20        30        40        50        60        70        80        90       100
nWASP      MSSVQQQPPPPRRVTNVGSLLLTPQENESLFTFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKdnpqrshFLRIFDIKDGKLLWEQELYNNFVY
All Expts                                                                                                     
C18_INHIBB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               

                  110       120       130       140       150       160       170       180       190       200
nWASP      NSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRrqRKSEKRRDPPNGPNLPMATVDIKNPEITTNRFYGPQVNNISHTKEKKKGKAKKKRLTK
All Expts                                                                                                     
C18_INHIBB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     

                  210       220       230       240       250       260       270       280       290       300
nWASP      GDIGTPSNFQHIGHVGWDPNTGsDLNNLDPELKNLFdmcgilEAQLKERETLKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRggpppppppphssgpp
All Expts                                                                                                     
C18_INHIBB                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                           

                  310       320       330       340       350       360       370       380       390       400
nWASP      pppargrgapppppsrAPTAAPPPPPPSRpsvevpppppnRMYPPPPPALPSSAPSGPPPPPPSVLGVGPVAPPPPPPPPPPPGPPPPPGLPSDGDHQVP
All Expts                                                                                                     
C18_INHIBB                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                           

                  410       420       430       440       450       460       470       480       490       500
nWASP      TTAGNkaaLLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVADGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDEDDEEDFEDD
All Expts                               * ?                       **  *                       **               
C18_INHIBB                              ? ?                                                   **                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                              KKVEQNSRPVSCSGRDALLDQIRQGIQL                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                      QKRSKAIHSSDEDEDEDDEEDFEDD                                                                                                                                                                                                                                                                                                                                                                                                                                    

                
nWASP      DEWED
All Expts      
C18_INHIBB      
                
           DEWED


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB
IMAC_INHIB
TIO_INHIB
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.