Phosphorylation of MLRC

Experimental Methods

MLRC phosphorylation site summary

Peptides - mlrc_c18_inhib.aln

              1       10        20        30        40        50        60        70        80        90       
MLC2       LGSMSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQEfkeAFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGT
All Expts                        *                                                                             
C18_INHIB                        *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
           LGSMSSKRAKAKTTKKRPQRATSNVFAMFDQSQIQE                                      GMMSEAPGPINFTMFLTMFGE                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                  AFNMIDQNRDGFIDKEDLHDMLASMGKNPTDEYLE                     KLNGT                                                                                                                                                                                                                                                                                                                                                                                                                                                                  

           100       110       120       130       140       150       160       170  
MLC2       DPEDVIRNAFACFDEEASGFIHEDHLRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKDD
All Expts                                       *       *            *                
C18_INHIB                                       *       *            *                                                                                                                                                                                                                                  
                           ASGFIHEDHLRE                MYREAPIDKKGNFNYVE                                                                                                                                                                                                                                
           DPEDVIRNAFACFDEE            LLTTMGDRFTDEEVDE                 FTRILKHGAKDKDD

Legend

* Phosphorylated sites are denoted by an asterisk below the amino acid residue.

[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.

All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.

C18_INHIB IMAC_INHIB TIO_INHIB Analysis type.

Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.

*``**	Phosphorylated sites are denoted by an asterisk below the amino acid residue.
`[ ]`	The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
`All Expts`	A summary of all phosphorylation sites detected on this molecule from all experiments.
`C18_INHIB IMAC_INHIB TIO_INHIB`	Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.