| Phosphoproteomics |
Phosphorylation of GIT1
| C18 (+) inhibitors (beads) | Alignment / Methods |
| IMAC (+) inhibitors (beads) | Alignment / Methods |
| C18 (+) inhibitors (beads dup) | Alignment / Methods |
| IMAC (+) inhibitors (beads dup) | Alignment / Methods |
| C18 (+) inhibitors (elut) | Alignment / Methods |
| IMAC (+) inhibitors (elut) | Alignment / Methods |
| Site | Position | Peptide | ||
|---|---|---|---|---|
| (+) Inhib | ||||
| 246Y | 243 - 254 | NGHYIIPQMADR | ||
| 261S | 259 - 271 | CMSQSLDLSELAK | ++ | |
| 293Y | 284 - 299 | LFEELAMDVYDEVDRR | ++ | |
| 370S-371S | 368 - 381 | SL[SS]PTDNLELSLR | +++ | |
| 370S-371S | 370 - 381 | [SS]PTDNLELSLR | ++ | |
| 371S | 358 - 378 | SEAKRRQQGKSLSSPTDNLEL | ||
| 371S | 368 - 378 | SLSSPTDNLEL | # | |
| 371S | 368 - 381 | SLSSPTDNLELSLR | +++ | |
| 373T | 368 - 381 | SLSSPTDNLELSLR | +++ | |
| 382S-384S / 392Y / 394S / 401T | 382 - 407 | [SQS]DLDDQHDYDSVASDEDTDQEPLR | ||
| 382S-384S / 394S / 397S | 382 - 407 | [SQS]DLDDQHDYDSVASDEDTDQEPLR | ||
| 384S / 394S / 397S | 382 - 407 | SQSDLDDQHDYDSVASDEDTDQEPLR | ||
| 384S / 394S / 397S / 401T | 382 - 407 | SQSDLDDQHDYDSVASDEDTDQEPLR | ||
| 384S / 397S | 382 - 407 | SQSDLDDQHDYDSVASDEDTDQEPLR | ++ | |
| 384S / 397S-401T | 382 - 407 | SQSDLDDQHDYDSVA[SDEDT]DQEPLR | +++ | |
| 392Y / 394S | 382 - 407 | SQSDLDDQHDYDSVASDEDTDQEPLR | +++ | |
| 392Y / 397S | 382 - 407 | SQSDLDDQHDYDSVASDEDTDQEPLR | +++ | |
| 392Y / 401T | 382 - 407 | SQSDLDDQHDYDSVASDEDTDQEPLR | +++ | |
| 394S / 401T | 382 - 407 | SQSDLDDQHDYDSVASDEDTDQEPLR | +++ | |
| 397S | 382 - 407 | SQSDLDDQHDYDSVASDEDTDQEPLR | +++ | |
| 419S | 414 - 435 | SNRARSMDSSDVSDGAVTLQEY | # | |
| 419S | 417 - 439 | ARSMDSSDVSDGAVTLQEYLELK | # | |
| 419S | 417 - 440 | ARSMDSSDVSDGAVTLQEYLELKK | ++++ | |
| 419S-426S | 417 - 439 | AR[SMDSSDVS]DGAVTLQEYLELK | +++ | |
| 422S-423S | 419 - 438 | SMD[SS]DVSDGAVTLQEYLEL | ||
| 457S-458S | 454 - 463 | KVN[SS]LSDEL | ||
| 489T | 482 - 496 | QPPGPVPTPPLPSER | +++ | |
| 507S-508T | 497 - 510 | AEHTPMAPGG[ST]HR | ||
| 517S-519Y | 512 - 539 | DRQAF[SMY]EPGSALKPFGGPPGDELTTR | +++ | |
| 519Y | 514 - 528 | QAFSMYEPGSALKPF | # | |
| 519Y | 514 - 529 | QAFSMYEPGSALKPFG | + | |
| 519Y | 514 - 539 | QAFSMYEPGSALKPFGGPPGDELTTR | +++ | |
| 519Y | 517 - 528 | SMYEPGSALKPF | # | |
| 545S / 554Y | 540 - 564 | LQPFHSTELEDDAIYSVHVPAGLYR | ||
| 545S-546T / 554Y-555S | 540 - 564 | LQPFH[ST]ELEDDAI[YS]VHVPAGLYR | ++ | |
| 545S-546T / 555S | 540 - 564 | LQPFH[ST]ELEDDAIYSVHVPAGLYR | ++ | |
| 545S-555S | 540 - 564 | LQPFH[STELEDDAIYS]VHVPAGLYR | ++++ | |
| 554Y | 540 - 554 | LQPFHSTELEDDAIY | +++ | |
| 554Y | 540 - 557 | LQPFHSTELEDDAIYSVH | ++ | |
| 554Y | 540 - 560 | LQPFHSTELEDDAIYSVHVPA | ||
| 554Y | 540 - 561 | LQPFHSTELEDDAIYSVHVPAG | ++ | |
| 554Y | 540 - 562 | LQPFHSTELEDDAIYSVHVPAGL | ++ | |
| 554Y | 540 - 564 | LQPFHSTELEDDAIYSVHVPAGLYR | ++++ | |
| 554Y | 544 - 562 | HSTELEDDAIYSVHVPAGL | # | |
| 554Y | 544 - 563 | HSTELEDDAIYSVHVPAGLY | # | |
| 554Y | 544 - 564 | HSTELEDDAIYSVHVPAGLYR | ++ | |
| 554Y | 551 - 560 | DAIYSVHVPA | ||
| 554Y | 551 - 562 | DAIYSVHVPAGL | ||
| 554Y | 551 - 563 | DAIYSVHVPAGLY | ||
| 554Y | 551 - 564 | DAIYSVHVPAGLYR | ||
| 554Y | 552 - 564 | AIYSVHVPAGLYR | ||
| 554Y-555S | 540 - 561 | LQPFHSTELEDDAI[YS]VHVPAG | +++ | |
| 554Y-555S | 544 - 563 | HSTELEDDAI[YS]VHVPAGLY | ||
| 554Y-555S | 551 - 561 | DAI[YS]VHVPAG | ||
| 555S | 540 - 563 | LQPFHSTELEDDAIYSVHVPAGLY | ++ | |
| 563Y | 540 - 564 | LQPFHSTELEDDAIYSVHVPAGLYR | ||
| 563Y | 555 - 564 | SVHVPAGLYR | ||
| 572S | 564 - 576 | RIRKGVSASAVPF | ||
| 594S-597S / 601S-607Y | 592 - 622 | HT[SKLS]RHG[SGADSDY]ENTQSGDPLLGLEGK | ||
| 601S | 597 - 616 | SRHGSGADSDYENTQSGDPL | # | |
| 601S | 599 - 608 | HGSGADSDYE | ||
| 601S | 599 - 611 | HGSGADSDYENTQ | ||
| 601S | 599 - 622 | HGSGADSDYENTQSGDPLLGLEGK | +++ | |
| 601S | 599 - 623 | HGSGADSDYENTQSGDPLLGLEGKR | ++ | |
| 601S / 605S | 599 - 609 | HGSGADSDYEN | ||
| 601S / 605S | 599 - 611 | HGSGADSDYENTQ | ||
| 601S / 605S | 599 - 622 | HGSGADSDYENTQSGDPLLGLEGK | +++ | |
| 601S / 605S | 599 - 623 | HGSGADSDYENTQSGDPLLGLEGKR | +++ | |
| 601S / 605S / 607Y | 599 - 622 | HGSGADSDYENTQSGDPLLGLEGK | # | |
| 601S / 605S-607Y | 599 - 622 | HGSGAD[SDY]ENTQSGDPLLGLEGK | +++ | |
| 601S-610T | 599 - 622 | HG[SGADSDYENT]QSGDPLLGLEGK | +++ | |
| 605S | 599 - 623 | HGSGADSDYENTQSGDPLLGLEGKR | # | |
| 605S / 607Y | 599 - 622 | HGSGADSDYENTQSGDPLLGLEGK | # | |
| 605S / 610T | 599 - 622 | HGSGADSDYENTQSGDPLLGLEGK | +++ | |
| 607Y | 597 - 624 | SRHGSGADSDYENTQSGDPLLGLEGKRF | # | |
| 607Y | 599 - 622 | HGSGADSDYENTQSGDPLLGLEGK | # | |
| 607Y | 599 - 623 | HGSGADSDYENTQSGDPLLGLEGKR | ||
| 639S | 624 - 663 | FLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTK | # | |
| 709S-710S | 701 - 712 | RPALEPVR[SS]LR | ||
| 771S | 769 - 780 | KQSTDYKDDDDK |
*Peptides detected only by
enrichment with
immobilized metal-affinity chromatography (IMAC).
# Peptides which do not require enrichment by IMAC for detection.