Experimental Methods

GIT1 phosphorylation site summary


Peptides - git1_imac_inh3.aln

                   10        20        30        40        50        60        70        80        90       100
GIT1_HUM   msrkgprAEVCADCSAPDPGWASISRGVLVCDECCSVHRslGRHISIVKHLrHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVH
All Expts                                                                                                      
IMAC_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                  110       120       130       140       150       160       170       180       190       200
GIT1_HUM   PIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKdlskqlhssvrTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNG
All Expts                                                                                                      
IMAC_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                  210       220       230       240       250       260       270       280       290       300
GIT1_HUM   RTPIDYARQAGHHELAERLVECQYELTDRLAFYlcgrKPDHKNGHYIIPQMADRsrQKCMSQSLDLSELAKaakKKLQALSNRLFEELAMDVYDEVDRRE
All Expts                                               *              *                               *       
IMAC_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

                  310       320       330       340       350       360       370       380       390       400
GIT1_HUM   NDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRnqgrqklarfnarEFATLIIDILSEAKRrqqgkSLSSPTDNLELSLRSQSDLDDQHDYDSVASDED
All Expts                                                                       [* *     ?  [ *       * *  *   
IMAC_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                          EFATLIIDILSEAK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

                  410       420       430       440       450       460       470       480       490       500
GIT1_HUM   TDQEPLRstgatrsnrARSMDSSDVSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAKNLQLRQPPGPVPTPPLPSERAEHT
All Expts  *                 *  []  ]                                                              *           
IMAC_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         

                  510       520       530       540       550       560       570       580       590       600
GIT1_HUM   PMAPGGSTHRrDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFtpssplpscsqegsrHTSKLSRHG
All Expts        []        [ *                         *]       **       *        *                     [  ]   
IMAC_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                             HG                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  

                  610       620       630       640       650       660       670       680       690       700
GIT1_HUM   SGADSDYENTQSGDPLLGLEGKrFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPK
All Expts  *   * *  *                            *                                                             
IMAC_INH3  *   * ]                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
           SGADSDYENTQSGDPLLGLEGKR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                  710       720       730       740       750       760       770       780
GIT1_HUM   RPALEPVRSSLRLLNASAYRlqsecrKTVPPEPGAPVDFQLLTQQVIQCAYDIAKaakQLVTITTRekKQSTDYKDDDDK
All Expts          []                                                            *         
IMAC_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB, C18,
IMAC_INHIB, IMAC
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.