Experimental Methods

GIT1 phosphorylation site summary


Peptides - git1_c18_inh3.aln

                   10        20        30        40        50        60        70        80        90       100
GIT1_HUM   msrkgprAEVCADCSAPDPGWASISRGVLVCDECCSVHRslGRHISIVKHLrHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVH
All Expts                                                                                                      
C18_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                  AEVCADCSAPDPGWASISR                          HSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGR                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                     GVLVCDECCSVHR                                                   RKANPQDKVH                                                                                                                                                                                                                                                                                                                                                                                                                                    

                  110       120       130       140       150       160       170       180       190       200
GIT1_HUM   PIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKdlskqlhssvrTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNG
All Expts                                                                                                      
C18_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                   AKYQMLAFVHK                       TGNLETCLR                         AGQTLQAELLVVYGADPGSPDVNG                                                                                                                                                                                                                                                                                                                                                                                                                                    
           PIKSEFIR           LPCRDDDGVTAK                    LLSLGAQANFFHPEK                                                                                                                                                                                                                                                                                                                                                                                                                                                                      

                  210       220       230       240       250       260       270       280       290       300
GIT1_HUM   RTPIDYARQAGHHELAERLVECQYELTDRLAFYlcgrKPDHKNGHYIIPQMADRsrQKCMSQSLDLSELAKaakKKLQALSNRLFEELAMDVYDEVDRRE
All Expts                                               *              *                               *       
C18_INH3                                                               *                                                                                                                                                                                                                                                                                                                                                                                                               
           R       QAGHHELAER                   KPDHKNGHYIIPQMADR                     KLQALSNR                E                                                                                                                                                                                                                                                                                                                                                                        
            TPIDYAR          LVECQYELTDR                           QKCMSQSLDLSELAK            LFEELAMDVYDEVDRR                                                                                                                                                                                                                                                                                                                                                                         

                  310       320       330       340       350       360       370       380       390       400
GIT1_HUM   NDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRnqgrqklarfnarEFATLIIDILSEAKRrqqgkSLSSPTDNLELSLRSQSDLDDQHDYDSVASDED
All Expts                                                                       [* *     ?  [ *       * *  *   
C18_INH3                                                                        [* *          *       * *  *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
           NDAVWLATQNHSTLVTER                             EFATLIIDILSEAKR                   SQSDLDDQHDYDSVASDED                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                             SAVPFLPVNPEYSATR                                 SLSSPTDNLELSLR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           

                  410       420       430       440       450       460       470       480       490       500
GIT1_HUM   TDQEPLRstgatrsnrARSMDSSDVSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAKNLQLRQPPGPVPTPPLPSERAEHT
All Expts  *                 *  []  ]                                                              *           
C18_INH3   *                 *                                                                     *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
           TDQEPLR                                 ALATSEAKVQQLMK           LQREIHKLQAK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                           ARSMDSSDVSDGAVTLQEYLELKK              VNSSLSDELR            NLQLRQPPGPVPTPPLPSER                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              

                  510       520       530       540       550       560       570       580       590       600
GIT1_HUM   PMAPGGSTHRrDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFtpssplpscsqegsrHTSKLSRHG
All Expts        []        [ *                         *]       **       *        *                     [  ]   
C18_INH3                   [ *                         []       **                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                      DRQAFSMYEPGSALKPFGGPPGDELTTR                                                           HG                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                  LQPFHSTELEDDAIYSVHVPAGLYR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      

                  610       620       630       640       650       660       670       680       690       700
GIT1_HUM   SGADSDYENTQSGDPLLGLEGKrFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPK
All Expts  *   * *  *                            *                                                             
C18_INH3   *   * ]                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
           SGADSDYENTQSGDPLLGLEGKR                                        NIQELLR                IHLAVTEMASLFPK                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                  FLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTK       AAQEFKHDSFVPCSEK                                                                                                                                                                                                                                                                                                                                                                                                                                                  

                  710       720       730       740       750       760       770       780
GIT1_HUM   RPALEPVRSSLRLLNASAYRlqsecrKTVPPEPGAPVDFQLLTQQVIQCAYDIAKaakQLVTITTRekKQSTDYKDDDDK
All Expts          []                                                            *         
C18_INH3                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                     KTVPPEPGAPVDFQLLTQQVIQCAYDIAK                                                                                                                                                                                                                                                                                                                                                                                                                                                             
           RPALEPVR                                                            KQSTDYKDDDDK                                                                                                                                                                                                                                                                                                                                                                                                                                    


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB, C18,
IMAC_INHIB, IMAC
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.