Experimental Methods

GIT1 phosphorylation site summary


Peptides - git1_c18_inh2.aln

                   10        20        30        40        50        60        70        80        90       100
GIT1_HUM   msrkgprAEVCADCSAPDPGWASISRGVLVCDECCSVHRslGRHISIVKHLrHSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGRRKANPQDKVH
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                  AEVCADCSAPDPGWASISR                          HSAWPPTLLQMVHTLASNGANSIWEHSLLDPAQVQSGR                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                      HISIVK                                          KANPQDKVH                                                                                                                                                                                                                                                                                                                                                                                                                                    

                  110       120       130       140       150       160       170       180       190       200
GIT1_HUM   PIKSEFIRAKYQMLAFVHKLPCRDDDGVTAKdlskqlhssvrTGNLETCLRLLSLGAQANFFHPEKGTTPLHVAAKAGQTLQAELLVVYGADPGSPDVNG
All Expts                                                                                                      
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                   AKYQMLAFVHK                                LLSLGAQANFFHPEK          AGQTLQAELLVVYGADPGSPDVNG                                                                                                                                                                                                                                                                                                                                                                                                                                    
           PIKSEFIR              RDDDGVTAK                                   GTTPLHVAAK                                                                                                                                                                                                                                                                                                                                                                                                                                                            

                  210       220       230       240       250       260       270       280       290       300
GIT1_HUM   RTPIDYARQAGHHELAERLVECQYELTDRLAFYlcgrKPDHKNGHYIIPQMADRsrQKCMSQSLDLSELAKaakKKLQALSNRLFEELAMDVYDEVDRRE
All Expts                                               *              *                               *       
C18_INH2                                                                                               *                                                                                                                                                                                                                                                                                                                                                                               
           R       QAGHHELAER                                          SQSLDLSELAK            LFEELAMDVYDEVDRR                                                                                                                                                                                                                                                                                                                                                                         
            TPIDYAR                                  NGHYIIPQMADR                    KKLQALSNR                E                                                                                                                                                                                                                                                                                                                                                                        

                  310       320       330       340       350       360       370       380       390       400
GIT1_HUM   NDAVWLATQNHSTLVTERSAVPFLPVNPEYSATRnqgrqklarfnarEFATLIIDILSEAKRrqqgkSLSSPTDNLELSLRSQSDLDDQHDYDSVASDED
All Expts                                                                       [* *     ?  [ *       * *  *   
C18_INH2                                                                        []            *         *  *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                             SAVPFLPVNPEYSATR                                 SLSSPTDNLELSLR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
           NDAVWLATQNHSTLVTER                             EFATLIIDILSEAKR                   SQSDLDDQHDYDSVASDED                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        

                  410       420       430       440       450       460       470       480       490       500
GIT1_HUM   TDQEPLRstgatrsnrARSMDSSDVSDGAVTLQEYLELKKALATSEAKVQQLMKVNSSLSDELRRLQREIHKLQAKNLQLRQPPGPVPTPPLPSERAEHT
All Expts  *                 *  []  ]                                                              *           
C18_INH2   *                 *      ]                                                              *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                           ARSMDSSDVSDGAVTLQEYLELKK              VNSSLSDELR                 QPPGPVPTPPLPSER                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
           TDQEPLR                                         VQQLMK           LQREIHKLQAK                    AEHT                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          

                  510       520       530       540       550       560       570       580       590       600
GIT1_HUM   PMAPGGSTHRrDRQAFSMYEPGSALKPFGGPPGDELTTRLQPFHSTELEDDAIYSVHVPAGLYRIRKGVSASAVPFtpssplpscsqegsrHTSKLSRHG
All Expts        []        [ *                         *]       **       *        *                     [  ]   
C18_INH2                     *                         [        **                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                        QAFSMYEPGSALKPFGGPPGDELTTR                            GVSASAVPF                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
           PMAPGGSTHR                             LQPFHSTELEDDAIYSVHVPAGLYR                                  HG                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  

                  610       620       630       640       650       660       670       680       690       700
GIT1_HUM   SGADSDYENTQSGDPLLGLEGKrFLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTKNIQELLRAAQEFKHDSFVPCSEKIHLAVTEMASLFPK
All Expts  *   * *  *                            *                                                             
C18_INH2   *   *    *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                  FLELGKEEDFHPELESLDGDLDPGLPSTEDVILKTEQVTK                       IHLAVTEMASLFPK                                                                                                                                                                                                                                                                                                                                                                                                                                    
           SGADSDYENTQSGDPLLGLEGKR                                        NIQELLR                                                                                                                                                                                                                                                                                                                                                                                                                                                                  

                  710       720       730       740       750       760       770       780
GIT1_HUM   RPALEPVRSSLRLLNASAYRlqsecrKTVPPEPGAPVDFQLLTQQVIQCAYDIAKaakQLVTITTRekKQSTDYKDDDDK
All Expts          []                                                            *         
C18_INH2                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                       LLNASAYR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
           RPALEPVR                                                  QLVTITTR                                                                                                                                                                                                                                                                                                                                                                                                                                                  


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB, C18,
IMAC_INHIB, IMAC
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.