Experimental Methods

FILA phosphorylation site summary


Peptides - fila_wt_lys2_c18.aln

                   10        20        30        40        50        60        70        80        90       100
FILA       mSSSHSRAGQSAAGAAPGGGVDTRDAEMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKcvskrianlqtdlsdglrliallevlsqkkmhrkhnqrptfr
All Expts            *                                                                                         
WT_LYS2_C1           *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
            SSSHSRAGQSAAGAAPGGGVDTRDAEMPATEKDLAEDAPWK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                     KIQQNTFTRWCNEHLK                                                                                                                                                                                                                                                                                                                                                                                                                                                                              

                  110       120       130       140       150       160       170       180       190       200
FILA       qmqlenvsvalefldresiklvsidskaivdgnlklilgliwtlilhysismpmwdeeedeeakkqtpkqrllGWIQNKLPQLPITNFSRDWQSGRALGA
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

                  210       220       230       240       250       260       270       280       290       300
FILA       LVDSCAPGLCPDWDSWDASKPVTNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKaKLKPGAPLRPKLNPKKARAYGPGIEPTGNMVKK
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                             KARAYGPGIEPTGNMVK                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                         

                  310       320       330       340       350       360       370       380       390       400
FILA       RAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNRTFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIANK
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                  VTVLFAGQHIAK         SQGDASKVTAQGPGLEPSGNIANK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                 NRTFSVWYVPEVTGTHK            SPFEVYVDK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      

                  410       420       430       440       450       460       470       480       490       500
FILA       TTYfeiftagagtgevevviqdpmgqkGTVEPQLEARGDSTYRCSYQPTMEGVHTVHVTFAGVPIPRSPYTVTVGQACNPSACravgrglqpkgvrvket
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

                  510       520       530       540       550       560       570       580       590       600
FILA       adfkvyTKGAGSGELKVTVKGPKGEERVKqkdlgdgvyGFEYYPMVPGTYivtitwGGQNIGRSPFEVKVGTECGNQKVRAWGPGLEGGVVGKSADFVVE
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                VGTECGNQK                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                   GAGSGELKVTVKGPKGEERVK                                                 VRAWGPGLEGGVVGK                                                                                                                                                                                                                                                                                                                                                                                                                                           

                  610       620       630       640       650       660       670       680       690       700
FILA       AIGDDVGTLGFSVEGPSQAKIEcddkgdgscdvrywpqeageyAVHVLCNSEDIRLSPFMADIRDAPQDFHPDRVKARGPGLEKTGVAVNKPAEFTVDAK
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                         IRDAPQDFHPDRVK        TGVAVNKPAEFTVDAK                                                                                                                                                                                                                                                                                                            
                                                                                       ARGPGLEK                                                                                                                                                                                                                                                                                                                            

                  710       720       730       740       750       760       770       780       790       800
FILA       HGGKAPLRVQVQDNEGCPVEALVKDNGNGTYSCSYVPRKPVKhtamvswGGVSIPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCAEAG
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                  VKVYGPGVAKTGLK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
               APLRVQVQDNEGCPVEALVKD                                                            AHEPTYFTVDCAEAG                                                                                                                                                                                                                                                                                                                                                                                                                                                                  

                  810       820       830       840       850       860       870       880       890       900
FILA       QGDVSIGIKCAPGVVGPAEADIDFDIIRNDNDTFTVKYTPRGAGSYtimvlfadqatptspirvKVEPSHDASKVKAEGPGLSRTGVELGKPTHFTVNAK
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                    CAPGVVGPAEADIDFDIIRNDNDTFTVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
           QGDVSIGIK                                                                 VKAEGPGLSRTGVELGKPTHFTVNAK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              

                  910       920       930       940       950       960       970       980       990      1000
FILA       AAGKGKLDVQFSGLTKGDAVRDVDIIDHHDNTYTVKYTPVQQGPVGVNVTYGGDPIPKSPFSVAVSPSLDLSKIKVSGLGEKVDVGKDQEFTVKSKGAGG
All Expts                                                                                             *        
WT_LYS2_C1                                                                                            *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
               GKLDVQFSGLTKGDAVRDVDIIDHHDNTYTVK                      SPFSVAVSPSLDLSKIK                   SKGAGG                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                               YTPVQQGPVGVNVTYGGDPIPK                 VSGLGEKVDVGKDQEFTVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    

                 1010      1020      1030      1040      1050      1060      1070      1080      1090      1100
FILA       QGKVASKIVGPSGAAVPCKVEPGLGADNSVVRFLPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVKAFGPGLQGGSAGSPARFTIDTKGAGTGGL
All Expts                                                                                     *    *           
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
           QGKVASK                                                                AFGPGLQGGSAGSPARFTIDTK                                                                                                                                                                                                                                                                                                                                                                                                                                           
                  IVGPSGAAVPCKVEPGLGADNSVVRFLPREEGPYEVEVTYDGVPVPGSPFPLEAVAPTKPSKVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                 1110      1120      1130      1140      1150      1160      1170      1180      1190      1200
FILA       GLTVEGPCEAQLECLDNGDGTCSVSYVPTEPGDYNINILFADTHIPGSPFKAHVVPCFDASKVKcsgpgleRATAGEVGQFqvdcssagsaeltieicse
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                                              AHVVPCFDASKVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  

                 1210      1220      1230      1240      1250      1260      1270      1280      1290      1300
FILA       aglpaevyIQDHGDGTHTITYIPLCPGAYTVTIKYGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVKARVANP
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                             YGGQPVPNFPSKLQVEPAVDTSGVQCYGPGIEGQGVFREATTEFSVDARALTQTGGPHVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                                                                         ARVANP                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            

                 1310      1320      1330      1340      1350      1360      1370      1380      1390      1400
FILA       SGNLTETYVQDRGDGMYKVEYTPYEEGLHSVDVTYDGSPVPSSPFqvpvtegcdPSRVRVHGPGIQSGTTNKPNKFTVETRGAGTGGLGLAVEGPSEAKM
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                 PSRVRVHGPGIQSGTTNKPNK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
           SGNLTETYVQDRGDGMYK                                                         FTVETRGAGTGGLGLAVEGPSEAK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                 1410      1420      1430      1440      1450      1460      1470      1480      1490      1500
FILA       SCMDNKDGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFKVPVHDVTDASKVKCSGPGLSPGMVRANLPQSFQVDTSKAGVAPLQVKVQGPKGLVEPVDVV
All Expts                            []                 [  *         *                                         
WT_LYS2_C1                                                           *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                 DGSCSVEYIPYEAGTYSLNVTYGGHQVPGSPFK             CSGPGLSPGMVRANLPQSFQVDTSK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                  VPVHDVTDASKVK                         AGVAPLQVKVQGPKGLVEPVDVV                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            

                 1510      1520      1530      1540      1550      1560      1570      1580      1590      1600
FILA       DNADGTQTVNYVPSREGPYSISVLYGDEEVPRSPFKVKVLPTHDASKVKASGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPEGKPKKTHIQDNH
All Expts                    [*    ]       *                                                                   
WT_LYS2_C1                   [     ]                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                          VKASGPGLNTTGVPASLPVEFTIDAK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
           DNADGTQTVNYVPSREGPYSISVLYGDEEVPRSPFK                                                        KTHIQDNH                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            

                 1610      1620      1630      1640      1650      1660      1670      1680      1690      1700
FILA       DGTYTVAYVPDVTGRYTILIKYGGDEIPFSPYRVRAVPTGDASKctvtvsigghglgagigptiqigeetvitvdtkaagkgkvtctvctpdgsevdvdv
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                YGGDEIPFSPYRVRAVPTGDASK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
           DGTYTVAYVPDVTGRYTILIK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                 1710      1720      1730      1740      1750      1760      1770      1780      1790      1800
FILA       venedgtfdifytapqpgkyVICVRFGGEHVPNSPFQVTALAGDQPSVQPPLRSQQLAPQYTYAQGGQQTWAPERPLVGVNGLDVTSLRPFDLVIPFtik
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                 1810      1820      1830      1840      1850      1860      1870      1880      1890      1900
FILA       KGEITGEVRMPSGKVAQPTITDNKDgtvtvryAPSEAGLHEMDIRYDNMHIPGSPLQFYvdyVNCGHVTAYGPGLTHGVVNKPATFTVNTKDAGEGGLSL
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                         VAQPTITDNKD                                                                  DAGEGGLSL                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
           KGEITGEVRMPSGK                                                                    PATFTVNTK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                 1910      1920      1930      1940      1950      1960      1970      1980      1990      2000
FILA       AIEGPSKAEISCTDNQDGTCSVSYLPVLPGDYSILVKYNEQHVPGSPFTARVTGDDSMRMSHLKVGSAADIPINISETDLSLLTATVVPPSGREEPCLLK
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
           AIEGPSK                              YNEQHVPGSPFTARVTGDDSMRMSHLK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                  AEISCTDNQDGTCSVSYLPVLPGDYSILVK                           VGSAADIPINISETDLSLLTATVVPPSGREEPCLLK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    

                 2010      2020      2030      2040      2050      2060      2070      2080      2090      2100
FILA       RLRNGHVGISFVPKETGEHLVHVKKngqhvasspipvvisqseigdasrvrvsgqglheghtfepaefIIDTRDAGYGGLSLsiegpskvdinteDLEDG
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       
           RLRNGHVGISFVPKETGEHLVHVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                 2110      2120      2130      2140      2150      2160      2170      2180      2190      2200
FILA       TCRVTYcptepgnyIINIKFADQHVPGSPFSVKVTGEGRVKESITRRRRAPSVANVGSHCDLSLKIPEISIQDMTAQVTSPSGKTHEAEIVEGENHTYCI
All Expts                                                     *                                                
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                            VTGEGRVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                              FADQHVPGSPFSVK                                IPEISIQDMTAQVTSPSGK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      

                 2210      2220      2230      2240      2250      2260      2270      2280      2290      2300
FILA       RFVPAEMGTHTVSVKYKGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFsiwtreagagglAIAVEGPSKAEISFEDRKDGSCGVAYVVQ
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                            GQHVPGSPFQFTVGPLGEGGAHK                                                 DGSCGVAYVVQ                                                                                                                                                                                                                                                                                                                                                                        
                                                                                           AEISFEDRK                                                                                                                                                                                                                                                                                                                                                                                   

                 2310      2320      2330      2340      2350      2360      2370      2380      2390      2400
FILA       EPGDYEVSVKFNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLKVNQPASFAVSLNGAKGAIDAKVHSPSGALEECYVTEIDQDKYAVRFIPRENGVY
All Expts                    *                                                                                 
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
           EPGDYEVSVK                                    VNQPASFAVSLNGAK                          YAVRFIPRENGVY                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                     FNEEHIPDSPFVVPVASPSGDARRLTVSSLQESGLK               GAIDAKVHSPSGALEECYVTEIDQDK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           

                 2410      2420      2430      2440      2450      2460      2470      2480      2490      2500
FILA       LIDVKFNGTHIPGSPFKirvgepghggdpglvsayGAGLEGGVTGNPAEFVVNTSNAGAGALsvtidgpskVKMDCQECPEGYRVTYTPMAPGSYLISIK
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
           LIDVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                FNGTHIPGSPFK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 

                 2510      2520      2530      2540      2550      2560      2570      2580      2590      2600
FILA       YGGPYHIGGSPFKAKVTGPRLVSNHSLHETSSVFVDSLTKATCAPQHGAPGPGPADASKVVAKGLGLSKAYVGQKSSFTVDCSKAGNNMLLVGVHGPRTP
All Expts                                                                                                      
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
           YGGPYHIGGSPFK                           ATCAPQHGAPGPGPADASKVVAKGLGLSK               AGNNMLLVGVHGPRTP                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                        AKVTGPRLVSNHSLHETSSVFVDSLTK                                   SSFTVDCSK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        

                 2610      2620      2630      2640       
FILA       CEEILVKHVGSRLYSVSYLLKDKGEYTLVVKwghehipgspyrvvvp
All Expts                                                 
WT_LYS2_C1                                                                                                                                                                                                                                                                                                                              
           CEEILVK              DKGEYTLVVK                                                                                                                                                                                                                                                                                              
                  HVGSRLYSVSYLLK                                                                                                                                                                                                                                                                                                        


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB
IMAC_INHIB
TIO_INHIB
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.