Experimental Methods

FAK phosphorylation site summary


Peptides - fak_imac_inhib.aln

                   10        20        30        40        50        60        70        80        90       100
FAK1_CHICK MAAAYLDPNLNHTPSSSAKTHLGTGMERSPGAMERVLKVFHYFENSSEPTTWASIIRHGDATDVRGIIQKIVDCHKVKnvacyglrlshlqseeVHWLHL
All_expts              *               *                                                                       
IMAC_INHIB                             *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                              THLGTGMERSPGAMERVLK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           

                  110       120       130       140       150       160       170       180       190       200
FAK1_CHICK DMGVSNVREkfelahppeewkyelrirylpkGFLNQFTEDKPTLNFFYQQVKNdymleiaDQVDQEIALKlgclEIRRSYGEMRGNALEKKSNYEVLEKd
All_expts                                                        *                                             
IMAC_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                                                                                                                                                                                                                                                                                                                                         

                  210       220       230       240       250       260       270       280       290       300
FAK1_CHICK vglrrffpkSLLDSVKaktlrkLIQQTFRQFANLNREESILKFFEILSPVYRFdkecfkCALGSSWIISVELAIGPEEGISYLTDKGANPTHLADFNQVQ
All_expts                                                                                                      
IMAC_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                  310       320       330       340       350       360       370       380       390       400
FAK1_CHICK TIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMAdliDGYCRLVNGATQSFIIRPQKeGERALPSIPKLANNEkQGVRSHTVSVSETDDYAEI
All_expts                                                                                       * * * * [  *   
IMAC_INHIB                                                                                      * * * *    *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                            QGVRSHTVSVSETDDYAEI                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            

                  410       420       430       440       450       460       470       480       490       500
FAK1_CHICK IDEEDTYTMPSTRdyEIQRERIELGRCIGEGQFGDVHQGIYMSPENPAMAVAIKtcknctsdsvrekFLQEALTMRQFdhphivkligvitenpvwiime
All_expts       **                                                                                             
IMAC_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
           IDEE                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    

                  510       520       530       540       550       560       570       580       590       600
FAK1_CHICK lctlgelrsflqvrkfsldlaslilyayqlstalayleskRFVHRDIAARNVLVSATDCVKLGDFGLSRYMEDSTYYKaskGKLPIKWMAPESINFRRFT
All_expts                                                                       *                              
IMAC_INHIB                                                                      *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                                                          DFGLSRYME                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     

                  610       620       630       640       650       660       670       680       690       700
FAK1_CHICK SASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKLQQeeRMRMESRRQVT
All_expts                                                                                                     *
IMAC_INHIB                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                           
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     

                  710       720       730       740       750       760       770       780       790       800
FAK1_CHICK VSWDSGGSDeappkpsrpgypsprsseGFYPSPQHMVQPNHYQVSGYSGSHGIPAMAGSIYPGQASLLDQTDSWNHRPQEvsawqpnmedsgtlDVRGMG
All_expts         *             *  []     *                                 *                          *       
IMAC_INHIB                      *  []                                       *                          *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         
                                                            SHGIPAMAGSIYPGQASLL                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                   DEAPPKPSRPGYPSPRSSEGF                                                            DSGTLDVRGMG                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  

                  810       820       830       840       850       860       870       880       890       900
FAK1_CHICK QVLPTHLMeerlirqqqemeeDQRWLEKEeRFLVMKPDVRLSRGSIEREDGGLQGPAGNQHIYQPVGKPDHAAPPKKPPRPGAPHLGSLASLNSPVDSYN
All_expts                                              *                                          *  *  *   ** 
IMAC_INHIB                                                                                        *  *  *   **                                                                                                                                                                                                                                                                               
                                                                                       KPPRPGAPHLGSLASLNSPVDSYN                                                                                                                                                                                                                                                                              
           QVLPTHLM                                                                                                                                                                                                                                                                                                                                                                          

                  910       920       930       940       950       960       970       980       990      1000
FAK1_CHICK EGVKIKPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDESLPVLPASTHREIEMAQKLLNSDLAELIN
All_expts            *                                                                                         
IMAC_INHIB           *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
           EGVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
               IKPQEISPPPTANL                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      

                 1010      1020      1030      1040      1050   
FAK1_CHICK KMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKnllDVIDQARLKmisqsrph
All_expts                                                       
IMAC_INHIB                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                  
                                                                                                                                                                                                                                                                                  


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB, C18,
IMAC_INHIB, IMAC
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.


Experimental Methods will be available shortly. Typical experimental methods are described for Paxillin.