Experimental Methods

FAK phosphorylation site summary


Peptides - fak_c18.aln

                   10        20        30        40        50        60        70        80        90       100
FAK1_CHICK MAAAYLDPNLNHTPSSSAKTHLGTGMERSPGAMERVLKVFHYFENSSEPTTWASIIRHGDATDVRGIIQKIVDCHKVKnvacyglrlshlqseeVHWLHL
All_expts              *               *                                                                       
C18                                    *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
               YLDPNLNHTPSSSAK                   VFHYFENSSEPTTWASIIRHGDAT                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                              THLGTGMERSPGAMERVLK                        DVRGIIQKIVDCHKVK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  

                  110       120       130       140       150       160       170       180       190       200
FAK1_CHICK DMGVSNVREkfelahppeewkyelrirylpkGFLNQFTEDKPTLNFFYQQVKNdymleiaDQVDQEIALKlgclEIRRSYGEMRGNALEKKSNYEVLEKd
All_expts                                                        *                                             
C18                                                                                                                                                                                                                                                                                                                                                                                                                                                      
                   EKFELAHPPEEWKY                                      DQVDQEIALK                    KSNYEVLEK                                                                                                                                                                                                                                                                                                                                           
                                          GFLNQFTEDKPTLNFFYQQVKN                     EIRRSYGEMRGNALEK                                                                                                                                                                                                                                                                                                                                                    

                  210       220       230       240       250       260       270       280       290       300
FAK1_CHICK vglrrffpkSLLDSVKaktlrkLIQQTFRQFANLNREESILKFFEILSPVYRFdkecfkCALGSSWIISVELAIGPEEGISYLTDKGANPTHLADFNQVQ
All_expts                                                                                                      
C18                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
                                                                                                 GANPTHLA                                                                                                                                                                                                                                                                                                                                                                                                                                          
                                                     FFEILSPVYRF                                         DFNQVQ                                                                                                                                                                                                                                                                                                                                                                                                                                    

                  310       320       330       340       350       360       370       380       390       400
FAK1_CHICK TIQYSNSEDKDRKGMLQLKIAGAPEPLTVTAPSLTIAENMAdliDGYCRLVNGATQSFIIRPQKeGERALPSIPKLANNEkQGVRSHTVSVSETDDYAEI
All_expts                                                                                       * * * * [  *   
C18                                                                                                        *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                     DRKGMLQLK                         DGYCRLVNGATQSFIIRPQK                 QGVRSHTVSVSETDDYAEI                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
           TIQYSNSEDK         IAGAPEPLTVTAPSLTIAENMA                       EGERALPSIPK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     

                  410       420       430       440       450       460       470       480       490       500
FAK1_CHICK IDEEDTYTMPSTRdyEIQRERIELGRCIGEGQFGDVHQGIYMSPENPAMAVAIKtcknctsdsvrekFLQEALTMRQFdhphivkligvitenpvwiime
All_expts       **                                                                                             
C18                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                
           IDEE                              DVHQGIYMSPENPAMAVAIK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                          EIQRERIELGRCIGEGQFG                                 FLQEALTMRQF                                                                                                                                                                                                                                                                                                                                                                                                                                                          

                  510       520       530       540       550       560       570       580       590       600
FAK1_CHICK lctlgelrsflqvrkfsldlaslilyayqlstalayleskRFVHRDIAARNVLVSATDCVKLGDFGLSRYMEDSTYYKaskGKLPIKWMAPESINFRRFT
All_expts                                                                       *                              
C18                                                                             *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                    
                                                        DIAARNVLVSATDCVK                    GKLPIKWMAPESINFRRFT                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
                                                                          DFGLSRYMEDSTYYK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            

                  610       620       630       640       650       660       670       680       690       700
FAK1_CHICK SASDVWMFGVCMWEILMHGVKPFQGVKNNDVIGRIENGERLPMPPNCPPTLYSLMTKCWAYDPSRRPRFTELKAQLSTILEEEKLQQeeRMRMESRRQVT
All_expts                                                                                                     *
C18                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
           SAS                        NNDVIGRIENGERLPMPPNCPPTLYSLMTK                           LQQEERMRM                                                                                                                                                                                                                                                                                                                                                                                                             
              DVWMFGVCMWEILMHGVKPFQGVK                                              AQLSTILEEEK                                                                                                                                                                                                                                                                                                                                                                                                                      

                  710       720       730       740       750       760       770       780       790       800
FAK1_CHICK VSWDSGGSDeappkpsrpgypsprsseGFYPSPQHMVQPNHYQVSGYSGSHGIPAMAGSIYPGQASLLDQTDSWNHRPQEvsawqpnmedsgtlDVRGMG
All_expts         *             *  []     *                                 *                          *       
C18                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              
                                                                                  DSWNHRPQEVSAWQPNMEDSGTL                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                   HYQVSGYSGSHGIPAMAGSIYPGQASLL                          DVRGMG                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  

                  810       820       830       840       850       860       870       880       890       900
FAK1_CHICK QVLPTHLMeerlirqqqemeeDQRWLEKEeRFLVMKPDVRLSRGSIEREDGGLQGPAGNQHIYQPVGKPDHAAPPKKPPRPGAPHLGSLASLNSPVDSYN
All_expts                                              *                                          *  *  *   ** 
C18                                                                                                                                                                                                                                                                                                                                                                                          
                                DQRWLEKE                                               KPPRPGAPHLGSLASLNSPVDSYN                                                                                                                                                                                                                                                                              
           QVLPTHLM                                         DGGLQGPAGNQHIYQPVGKPDHAAPPK                                                                                                                                                                                                                                                                                                      

                  910       920       930       940       950       960       970       980       990      1000
FAK1_CHICK EGVKIKPQEISPPPTANLDRSNDKVYENVTGLVKAVIEMSSKIQPAPPEEYVPMVKEVGLALRTLLATVDESLPVLPASTHREIEMAQKLLNSDLAELIN
All_expts            *                                                                                         
C18                  *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
           EGVK              DRSNDKVYENVTGLVK                      EVGLALRTLLATV                    LLNSDLAELIN                                                                                                                                                                                                                                                                                                                                                                                                                                    
               IKPQEISPPPTANL                AVIEMSSKIQPAPPEEYVPMVK             DESLPVLPASTHREIEMAQK                                                                                                                                                                                                                                                                                                                                                                                                                                               

                 1010      1020      1030      1040      1050   
FAK1_CHICK KMKLAQQYVMTSLQQEYKKQMLTAAHALAVDAKnllDVIDQARLKmisqsrph
All_expts                                                       
C18                                                                                                                                                                                                                                                                               
           K                                   DVIDQARLK                                                                                                                                                                                                                          
            MKLAQQYVMTSLQQEYKK                                                                                                                                                                                                                                                    


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB, C18,
IMAC_INHIB, IMAC
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.