Experimental Methods

Cortactin phosphorylation site summary


Peptides - cort_imac_T.aln

                   10        20        30        40        50        60        70        80        90       100
CORTACTIN  mwkASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYggkFGVEQDRMDRSAV
All Expts            * *          *                                                                            
IMAC_T                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
             KASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRW                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                          QEHQTLKEKELETGPKASHGY                                                                                                                                                                                                                                                                                                                                                                                        

                  110       120       130       140       150       160       170       180       190       200
CORTACTIN  GHEYQSKlskHCSQVDSVRGFGGKfGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFggkyG
All Expts                                                                         *                            
IMAC_T                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                                                              G                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       

                  210       220       230       240       250       260       270       280       290       300
CORTACTIN  IDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSSAVGFDYKERLAKHEPQQ
All Expts          *                                                                         *                 
IMAC_T                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
           IDKDKVDKSAVGF                       GVQTDRQDKCALGW                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                        EYQGKTEKHESQKDY                                                           DYKERLAKHEPQQ                                                                                                                                                                                                                                                                                                                                                                                                      

                  310       320       330       340       350       360       370       380       390       400
CORTACTIN  DYAKGFGGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFenlakereqedrrkaeaeraqrmakERQEQEEARRKLEEQARAKKQ
All Expts                       []                        *                                                    
IMAC_T                                                    *                                                                                                                                                                                                                                                                                                                                                                                              
                     GVQKDRMDKNASTFEEVVQVPSAY                                                                                                                                                                                                                                                                                                                                                                                                            
           DY                                QKTVPIEAVTSKTSNIRANF                                                                                                                                                                                                                                                                                                                                                                                        

                  410       420       430       440       450       460       470       480       490       500
CORTACTIN  TPPASPSPQPIEDRPPSSPIYEDAAPFKAEPSYRGSEPEPEYSIEAAGIPEAGSQQGLTYTSEPVYETTEAPGHYQAEDDTYDGYESDLGITAIALYdyq
All Expts        *         []  ]                                *                                              
IMAC_T                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                      KAEPSYRGSEPEPEYSIEAAGIPEAGSQQGLTY                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         

                  510       520       530       540      
CORTACTIN  aagddeisFDPDDIITNIEMIDDGWwrgvckgryGLFPANYVELRQ
All Expts                                                
IMAC_T                                                                                                                                                                           
                                                                                                                                                                                 
                                     RGVCKGRY                                                                                                                                    


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB, C18,
IMAC_INHIB, IMAC
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.