10 20 30 40 50 60 70 80 90 100
CORTACTIN mwkASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYggkFGVEQDRMDRSAV
All Expts * * *
C18_INHIB * *
ASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAK FGVEQDRMDRSAV
LRENVFQEHQTLKEKELETGPK
110 120 130 140 150 160 170 180 190 200
CORTACTIN GHEYQSKlskHCSQVDSVRGFGGKfGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFggkyG
All Expts *
C18_INHIB
GHEYQSK FGVQMDRVDQSAVGFEYQGKTEK DYQGKTEKHESQKDY
HCSQVDSVRGFGGK DYSSGFGGKYGVQADRVDK
210 220 230 240 250 260 270 280 290 300
CORTACTIN IDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSSAVGFDYKERLAKHEPQQ
All Expts * *
C18_INHIB
GFGGKFGVQTDRQDK FGVQSERQDSSAVGFDYKERLAKHEPQQ
SAVGFEYQGKTEKHESQK CALGWDHQEKLQLHESQK
310 320 330 340 350 360 370 380 390 400
CORTACTIN DYAKGFGGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFenlakereqedrrkaeaeraqrmakERQEQEEARRKLEEQARAKKQ
All Expts [] *
C18_INHIB []
DYAK NASTFEEVVQVPSAYQK KQ
GFGGKYGVQK TSNIRANFENLAK
410 420 430 440 450 460 470 480 490 500
CORTACTIN TPPASPSPQPIEDRPPSSPIYEDAAPFKAEPSYRGSEPEPEYSIEAAGIPEAGSQQGLTYTSEPVYETTEAPGHYQAEDDTYDGYESDLGITAIALYdyq
All Expts * [] ] *
C18_INHIB * [ ] *
TPPASPSPQPIEDRPPSSPIYEDAAPF TSEPVYETTEAPGHY
KAEPSYRGSEPEPEYSIEAAGIPEAGSQQGLTY
510 520 530 540
CORTACTIN aagddeisFDPDDIITNIEMIDDGWwrgvckgryGLFPANYVELRQ
All Expts
C18_INHIB
GRYGLFPANY
FDPDDIITNIEMIDDGW
Legend
| * | Phosphorylated sites are denoted by an asterisk below the amino acid residue. |
| [ ] | The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra. |
| All Expts | A summary of all phosphorylation sites detected on this molecule from all experiments. |
| C18_INHIB, C18, IMAC_INHIB, IMAC | Analysis type. |
|
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry. |
|
Experimental Methods will be available shortly. Typical experimental methods are described for Paxillin.