Experimental Methods

Cortactin phosphorylation site summary


Peptides - cort_c18_inhV.aln

                   10        20        30        40        50        60        70        80        90       100
CORTACTIN  mwkASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQEHINIHKLRENVFQEHQTLKEKELETGPKASHGYggkFGVEQDRMDRSAV
All Expts            * *          *                                                                            
C18_INHV                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
             KASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRW                                                 GVEQDRMDRSAV                                                                                                                                                                                                                                                                                                                                                                        
                                                                          QEHQTLKEKELETGPKASHGY                                                                                                                                                                                                                                                                                                                                                                                        

                  110       120       130       140       150       160       170       180       190       200
CORTACTIN  GHEYQSKlskHCSQVDSVRGFGGKfGVQMDRVDQSAVGFEYQGKTEKHASQKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFggkyG
All Expts                                                                         *                            
C18_INHV                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
           GHEY                                                                        DYQGKTEKHESQKDYSKGF                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                    GVQMDRVDQSAVGFEY                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   

                  210       220       230       240       250       260       270       280       290       300
CORTACTIN  IDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYKTGFGGKFGVQSERQDSSAVGFDYKERLAKHEPQQ
All Expts          *                                                                         *                 
C18_INHV                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             
                                           GGKFGVQTDRQDKCALGW                   GGKFGVQSERQDSSAVGF                                                                                                                                                                                                                                                                                                                                                                                                                   
                        EYQGKTEKHESQKDYVKGF                  DHQEKLQLHESQKDY                      DYKERLAKHEPQQ                                                                                                                                                                                                                                                                                                                                                                                                      

                  310       320       330       340       350       360       370       380       390       400
CORTACTIN  DYAKGFGGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNIRANFenlakereqedrrkaeaeraqrmakERQEQEEARRKLEEQARAKKQ
All Expts                       []                        *                                                    
C18_INHV                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                   EEVVQVPSAYQKTVPIEAVTSKTSNIRANF                                                                                                                                                                                                                                                                                                                                                                                        
           DYAKGF                                                                                                                                                                                                                                                                                                                                                                                                                                        

                  410       420       430       440       450       460       470       480       490       500
CORTACTIN  TPPASPSPQPIEDRPPSSPIYEDAAPFKAEPSYRGSEPEPEYSIEAAGIPEAGSQQGLTYTSEPVYETTEAPGHYQAEDDTYDGYESDLGITAIALYdyq
All Expts        *         []  ]                                *                                              
C18_INHV                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
                                                                       TSEPVYETTEAPGHY                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
                                      KAEPSYRGSEPEPEYSIEAAGIPEAGSQQGLTY               QAEDDTYDGYESDLGITAIALY                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                             

                  510       520       530       540      
CORTACTIN  aagddeisFDPDDIITNIEMIDDGWwrgvckgryGLFPANYVELRQ
All Expts                                                
C18_INHV                                                                                                                                                                         
                                             GLFPANYVELRQ                                                                                                                        
                                                                                                                                                                                 


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB, C18,
IMAC_INHIB, IMAC
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.