Experimental Methods

BPIX phosphorylation site summary


Peptides - bpix_c18_pdgf.aln

                                        1       10        20        30        40        50        60        70 
bPix       DYKDDDDKGPKLGTELGSTSNGRQCAGIRMTDNTNSQLVVRAKFNFQQTNEDELSFSKGDVIHVTRveeggwwegthngrTGWFPSNYVREIKPSEKPVS
All Expts                                                                                                     *
C18_PDGF                                                                                                      *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
            YKDDDDK                     MTDNTNSQLVVR                                       TGWFPSNY                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
                      LGTELGSTSNGR                  AKFNFQQTNEDELSFSKGDVIHVTR                        EIKPSEKPVS                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            

                  80        90       100       110       120       130       140       150       160       170 
bPix       PKSGTLKSPPKGFDTTAINKSYYNVVLQNILETEHEYSKELQSVLSTYLRPLQTSDKLSSANTSYLMGNLEEISSFQQVLVQSLEECTKSPEAQQRVGGC
All Expts         *                                                   *   *         **                         
C18_PDGF          *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                      
             SGTLKSPPKGFDTTAINK                   ELQSVLSTYLRPLQTSDK                                       VGGC                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
           PK                  SYYNVVLQNILETEHEYSK                  LSSANTSYLMGNLEEISSFQQVLVQSLEECTKSPEAQQR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                              

                 180       190       200       210       220       230       240       250       260       270 
bPix       FLSLMPQMRTLYLAYCANHPSAVSVLTEHSEDLGEFMETKGASSPGILVLTTGLSKPFMRLDKYPTLLKELERHMEDYHPDRQDIQKSMTAFKNLSAQCQ
All Expts                      *  *                                                                            
C18_PDGF                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
           FLSLMPQMR                               GASSPGILVLTTGLSKPFMR             HMEDYHPDR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          
                    TLYLAYCANHPSAVSVLTEHSEDLGEFMETK                    LDKYPTLLKELER              SMTAFKNLSAQCQ                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        

                 280       290       300       310       320       330       340       350       360       370 
bPix       EVRkrkELELQILTEPIRSWEGDDIKTLGSVTYMSQVTIQCAGSEEKNERylllfpnlllmlsasprMSGFIYQGKLPTTGMTITKLEDSENHRNAFEIS
All Expts                                                                      *                               
C18_PDGF                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                               
                 ELELQILTEPIRSWEGDDIKTLGSVTYMSQVTIQCAGSEEKNER                 MSGFIYQGKLPTTGMTITKLEDSENHRNAFEIS                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        
           EVR                                               YLLLFPNLLLMLSASPR                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         

                 380       390       400       410       420       430       440       450       460       470 
bPix       GSMIERILVSCTSQQDLHEWVEHLQKqtkVTSVSNPTIKPHSVPSHTLPSHPLTPSSKHADSKPVALTPAYHTLphpshhgtphttiswGPLEPPKTPKP
All Expts                                                         []                                           
C18_PDGF                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   
           GSMIER                       VTSVSNPTIKPHSVPSHTLPSHPLTPSSK                               GPLEPPKTPKP                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                            
                 ILVSCTSQQDLHEWVEHLQK                                HADSKPVALTPAY                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         

                 480       490       500       510       520       530       540       550       560       570 
bPix       WSLsclrPAPPLRPSAALCYKEDLSKSPKtmkkllpkRKPERKPSDEEFAVRKSTAALEEDAQILKVIEAYCTSAKtrqtlnsssrKESAPQVLLPEEEK
All Expts                            *                 *                         *                 *           
C18_PDGF                                               *                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
           WSL                                                  STAALEEDAQILKVIEAYCTSAK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                  
                                                     KPSDEEFAVRK                                 KESAPQVLLPEEEK                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                          

                 580       590       600       610       620       630       640      
bPix       IIVEETKSNGQTVIEEKSLVDTVYALKDEVQELRQDNKKmkKSLEEEQRARKDLEKLVRKVLKNMNDPAWDETNL
All Expts                   *   *                                                     
C18_PDGF                                                                                                                                                                                                                                                                                                                              
                            SLVDTVYALKDEVQELRQDNKK                    KVLKNMNDPAWDETNL                                                                                                                                                                                                                                                
           IIVEETKSNGQTVIEEK                         SLEEEQRAR                                                                                                                                                                                                                                                                        


Legend
*Phosphorylated sites are denoted by an asterisk below the amino acid residue.
[ ] The specific phosphorylated amino acid between the brackets can not be distinguished by the MS/MS spectra.
All Expts A summary of all phosphorylation sites detected on this molecule from all experiments.
C18_INHIB, C18,
IMAC_INHIB, IMAC
Analysis type.
Alternating, aligned peptides represent protein coverage for this experiment
Lower case amino acids have yet to be detected in any enzymatic digest analyzed by mass spectrometry.