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Transgenic & Knockout Mice - Protocols

Transgenic & Knockout Mice protocols describe step-by-step approaches to producing cell and mice with migration related defects. These have proved very popular protocols and can also still be accessed from their original locations in this Initiative's Resource pages.

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Karyotyping

Author(s): Noranne Enzer, Aurora Burds Connor, and Richard Hynes Lab/Group: - Publication date: November 2005

Construct Linearization

Author(s): Kayoko Kimbabra, Aurora Burds Connor, and Richard Hynes Lab/Group: - Publication date: August 2004

Beginner's Guide to Gene Targeting

Author(s): Aurora Burds Connor and Richard Hynes Lab/Group: Hynes Laboratory Publication date: -

Blastocyst Harvest

Author(s): Aurora Burds Connor, Max Dobles, and Richard Hynes Lab/Group: Hynes Laboratory Publication date: -

Knockout Mouse Production Time Line

Author(s): Aurora Burds Connor and Richard Hynes Lab/Group: Hynes Laboratory Publication date: -

Making Hot Probes for Southern Blots

Author(s): Aurora Burds Connor and Richard Hynes Lab/Group: Hynes Laboratory Publication date: -

MEF Protocol

Author(s): Aurora Burds Connor and Richard Hynes Lab/Group: Hynes Laboratory Publication date: -

Mouse Management

Author(s): Aurora Burds Connor and Richard Hynes Lab/Group: Hynes Laboratory Publication date: -

Southern Blot Protocol for alkaline transfer

Author(s): Aurora Burds Connor and Richard Hynes Lab/Group: Hynes Laboratory Publication date: -

Southern Blot Protocol for Neutral Transfer

Author(s): Elaine Pinheiro, Aurora Burds Connor, and Richard Hynes Lab/Group: Hynes Laboratory Publication date: -