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Imaging & Photomanipulation - Resources

Publications - last updated February, 2016

2013 | 2012 | 2011 | 2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Bachir AI, Kubow KE, Horwitz AR. Fluorescence fluctuation approaches to the study of adhesion and signaling. Meth. Enzymol. 2013; 519:167-201 PubMed.
  • Beaty BT, Sharma VP, Bravo-Cordero JJ, Simpson MA, Eddy RJ, Koleske AJ, Condeelis J. β1 integrin regulates Arg to promote invadopodial maturation and matrix degradation. Mol. Biol. Cell 2013; PubMed | CMC Update Article.
  • Bravo-Cordero JJ, Magalhaes MA, Eddy RJ, Hodgson L, Condeelis J. Functions of cofilin in cell locomotion and invasion. Nat. Rev. Mol. Cell Biol. 2013; 14:405-17 PubMed.
  • Bravo-Cordero JJ, Sharma VP, Roh-Johnson M, Chen X, Eddy R, Condeelis J, Hodgson L. Spatial regulation of RhoC activity defines protrusion formation in migrating cells. J. Cell. Sci. 2013; PubMed | CMC Update Article.
  • Chen Z, Lessey E, Berginski ME, Cao L, Li J, Trepat X, Itano M, Gomez SM, Kapustina M, Huang C, Burridge K, Truskey G, Jacobson K. Gleevec, an abl family inhibitor, produces a profound change in cell shape and migration. PLoS ONE 2013; 8:e52233 Full Text | CMC Update Article.
  • Chiu CL, Digman MA, Gratton E. Measuring Actin Flow in 3D Cell Protrusions. Biophys. J. 2013; 105:1746-55 Full Text | CMC Update Article.
  • Conway DE, Breckenridge MT, Hinde E, Gratton E, Chen CS, Schwartz MA. Fluid Shear Stress on Endothelial Cells Modulates Mechanical Tension across VE-Cadherin and PECAM-1. Curr. Biol. 2013; 23:1024-30 Full Text | CMC Update Article.
  • Digman MA, Stakic M, Gratton E. Raster image correlation spectroscopy and number and brightness analysis. Meth. Enzymol. 2013; 518:121-44 PubMed.
  • Hinde E, Digman MA, Hahn KM, Gratton E. Millisecond spatiotemporal dynamics of FRET biosensors by the pair correlation function and the phasor approach to FLIM. Proc. Natl. Acad. Sci. U.S.A. 2013; 110:135-40 Full Text | CMC Update Article.
  • Kapustina M, Elston TC, Jacobson K. Compression and dilation of the membrane-cortex layer generates rapid changes in cell shape. J. Cell Biol. 2013; 200:95-108 Full Text | CMC Update Article.
  • Kubow KE, Conrad SK, Horwitz AR. Matrix Microarchitecture and Myosin II Determine Adhesion in 3D Matrices. Curr. Biol. 2013; 23:1607-19 Full Text | CMC Update Article.
  • Sainlos M, Iskenderian-Epps WS, Olivier NB, Choquet D, Imperiali B. Caged Mono- and Divalent Ligands for Light-Assisted Disruption of PDZ Domain-Mediated Interactions. J. Am. Chem. Soc. 2013; PubMed | CMC Update Article.
  • Tejera E, Rocha-Perugini V, López-Martín S, Pérez-Hernández D, Bachir AI, Horwitz AR, Vázquez J, Sánchez-Madrid F, Yáñez-Mo M. CD81 regulates cell migration through its association with Rac GTPase. Mol. Biol. Cell 2013; 24:261-73 Full Text | CMC Update Article.

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  • Chen L, Vicente-Manzanares M, Potvin-Trottier L, Wiseman PW, Horwitz AR. The Integrin-Ligand Interaction Regulates Adhesion and Migration through a Molecular Clutch. PLoS ONE 2012; 7:e40202 Full Text | CMC Update Article.
  • Digman MA, Gratton E. Scanning image correlation spectroscopy. Bioessays 2012; PubMed.
  • Hinde E, Cardarelli F, Digman MA, Gratton E. Changes in chromatin compaction during the cell cycle revealed by micrometer-scale measurement of molecular flow in the nucleus. Biophys. J. 2012; 102:691-7 Full Text.
  • Itano MS, Steinhauer C, Schmied JJ, Forthmann C, Liu P, Neumann AK, Thompson NL, Tinnefeld P, Jacobson K. Super-resolution imaging of C-type lectin and influenza hemagglutinin nanodomains on plasma membranes using blink microscopy. Biophys. J. 2012; 102:1534-42 Full Text | CMC Update Article.
  • Kniazeva E, Weidling JW, Singh R, Botvinick EL, Digman MA, Gratton E, Putnam AJ. Quantification of local matrix deformations and mechanical properties during capillary morphogenesis in 3D. Integr Biol (Camb) 2012; 4:431-9 PubMed.
  • Liu P, Wang X, Itano MS, Neumann AK, Jacobson K, Thompson NL. The formation and stability of DC-SIGN microdomains require its extracellular moiety. Traffic 2012; 13:715-26 PubMed | CMC Update Article.
  • Stains CI, Tedford NC, Walkup TC, Luković E, Goguen BN, Griffith LG, Lauffenburger DA, Imperiali B. Interrogating signaling nodes involved in cellular transformations using kinase activity probes. Chem. Biol. 2012; 19:210-7 Full Text | CMC Update Article.
  • Toplak T, Pandzic E, Chen L, Vicente-Manzanares M, Horwitz AR, Wiseman PW. STICCS Reveals Matrix-Dependent Adhesion Slipping and Gripping in Migrating Cells. Biophys. J. 2012; 103:1672-82 Full Text | CMC Update Article.
  • Trepat X, Chen Z, Jacobson K. Cell Migration. Compr Physiol 2012; 2:2369-2392 PubMed.

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  • Bravo-Cordero JJ, Oser M, Chen X, Eddy R, Hodgson L, Condeelis J. A Novel Spatiotemporal RhoC Activation Pathway Locally Regulates Cofilin Activity at Invadopodia. Curr. Biol. 2011; 21:635-44 Full Text | CMC Update Article.
  • Choi CK, Zareno J, Digman MA, Gratton E, Horwitz AR. Cross-correlated fluctuation analysis reveals phosphorylation-regulated paxillin-FAK complexes in nascent adhesions. Biophys. J. 2011; 100:583-92 Full Text | CMC Update Article.
  • Goguen BN, Aemissegger A, Imperiali B. Sequential Activation and Deactivation of Protein Function Using Spectrally Differentiated Caged Phosphoamino Acids. J Am Chem Soc 2011; PubMed | CMC Update Article.
  • Goguen BN, Imperiali B. Chemical Tools for Studying Directed Cell Migration. ACS Chem Biol 2011; PubMed.
  • Hanein D, Horwitz AR. The structure of cell-matrix adhesions: the new frontier. Curr Opin Cell Biol 2011; PubMed.
  • Hinde E, Cardarelli F, Digman MA, Kershner A, Kimble J, Gratton E. The impact of mitotic versus interphase chromatin architecture on the molecular flow of EGFP by pair correlation analysis. Biophys. J. 2011; 100:1829-36 Full Text.
  • Hinde E, Digman MA, Welch C, Hahn KM, Gratton E. Biosensor Förster resonance energy transfer detection by the phasor approach to fluorescence lifetime imaging microscopy. Microsc Res Tech 2011; PubMed | CMC Update Article.
  • Huttenlocher A, Horwitz AR. Integrins in cell migration. Cold Spring Harb Perspect Biol 2011; 3:a005074 PubMed.
  • Kubow KE, Horwitz AR. Reducing background fluorescence reveals adhesions in 3D matrices. Nat. Cell Biol. 2011; 13:3-5; author reply 5-7 PubMed | CMC Update Article.
  • Lanzano L, Digman MA, Fwu P, Giral H, Levi M, Gratton E. Nanometer-scale imaging by the modulation tracking method. J Biophotonics 2011; 4:415-24 PubMed.
  • Marchini C, Pozzi D, Montani M, Alfonsi C, Amici A, Candeloro De Sanctis S, Digman MA, Sanchez S, Gratton E, Amenitsch H, Fabbretti A, Gualerzi CO, Caracciolo G. Role of temperature-independent lipoplex-cell membrane interactions in the efficiency boost of multicomponent lipoplexes. Cancer Gene Ther. 2011; 18:543-52 PubMed.
  • Stafford RL, Hinde E, Knight MJ, Pennella MA, Ear J, Digman MA, Gratton E, Bowie JU. Tandem SAM domain structure of human Caskin1: a presynaptic, self-assembling scaffold for CASK. Structure 2011; 19:1826-36 Full Text.
  • Vetri V, Ossato G, Militello V, Digman MA, Leone M, Gratton E. Fluctuation methods to study protein aggregation in live cells: concanavalin A oligomers formation. Biophys. J. 2011; 100:774-83 Full Text.
  • Vicente-Manzanares M, Horwitz AR. Adhesion dynamics at a glance. J. Cell. Sci. 2011; 124:3923-7 PubMed.
  • Vicente-Manzanares M, Horwitz AR. Cell migration: an overview. Methods Mol. Biol. 2011; 769:1-24 PubMed.
  • Vicente-Manzanares M, Newell-Litwa K, Bachir AI, Whitmore LA, Horwitz AR. Myosin IIA/IIB restrict adhesive and protrusive signaling to generate front-back polarity in migrating cells. J. Cell Biol. 2011; 193:381-96 Full Text | CMC Update Article.

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  • Cirit M, Krajcovic M, Choi CK, Welf ES, Horwitz AF, Haugh JM. Stochastic model of integrin-mediated signaling and adhesion dynamics at the leading edges of migrating cells. PLoS Comput. Biol. 2010; 6:e1000688 Full Text | CMC Update Article.
  • Costigliola N, Kapustina MT, Weinreb GE, Monteith A, Rajfur Z, Elston TC, Jacobson K. RhoA regulates calcium-independent periodic contractions of the cell cortex. Biophys. J. 2010; 99:1053-63 Full Text | CMC Update Article.
  • Digman MA, Gratton E. Lessons in Fluctuation Correlation Spectroscopy. Annu Rev Phys Chem 2010; PubMed.
  • Hinde E, Cardarelli F, Digman MA, Gratton E. In vivo pair correlation analysis of EGFP intranuclear diffusion reveals DNA-dependent molecular flow. Proc. Natl. Acad. Sci. U.S.A. 2010; 107:16560-5 Full Text.
  • Huang C, Jacobson K. Detection of protein-protein interactions using nonimmune IgG and BirA-mediated biotinylation. BioTechniques 2010; 49:881-6 PubMed | CMC Update Article.
  • Jacobson, K. Fluorescence Microscopy. In: Encyclopedia of Life Sciences (ELS). 2010; John Wiley & Sons, Ltd; Chichester: DOI.
  • Kapustina M, Vitriol E, Elston TC, Loew LM, Jacobson K. Modeling capping protein FRAP and CALI experiments reveals in vivo regulation of actin dynamics. Cytoskeleton (Hoboken) 2010; 67:519-34 Full Text | CMC Update Article.
  • Kim SA, Sanabria H, Digman MA, Gratton E, Schwille P, Zipfel WR, Waxham MN. Quantifying translational mobility in neurons: comparison between current optical techniques. J. Neurosci. 2010; 30:16409-16 Full Text.
  • Moens PD, Gratton E, Salvemini IL. Fluorescence correlation spectroscopy, raster image correlation spectroscopy, and number and brightness on a commercial confocal laser scanning microscope with analog detectors (Nikon C1). Microsc. Res. Tech. 2010; Full Text.
  • Neumann, AK, Itano, M, Jacobson, K. Understanding lipid rafts and other related membrane domains.. F1000 Biology Reports 2010; 2:31 DOI.
  • Neumann AK, Jacobson K. A novel pseudopodial component of the dendritic cell anti-fungal response: the fungipod. PLoS Pathog. 2010; 6:e1000760 Full Text | CMC Update Article.
  • Ossato G, Digman MA, Aiken C, Lukacsovich T, Marsh JL, Gratton E. A two-step path to inclusion formation of huntingtin peptides revealed by number and brightness analysis. Biophys. J. 2010; 98:3078-85 Full Text | CMC Update Article.
  • Parsons JT, Horwitz AR, Schwartz MA. Cell adhesion: integrating cytoskeletal dynamics and cellular tension. Nat. Rev. Mol. Cell Biol. 2010; 11:633-43 Full Text.
  • Rossow MJ, Sasaki JM, Digman MA, Gratton E. Raster image correlation spectroscopy in live cells. Nat Protoc 2010; 5:1761-74 PubMed | CMC Update Article.
  • Vicente-Manzanares M, Horwitz AR. Myosin light chain mono- and di-phosphorylation differentially regulate adhesion and polarity in migrating cells. Biochem. Biophys. Res. Commun. 2010; 402:537-42 Full Text | CMC Update Article.

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  • Caracciolo G, Caminiti R, Digman MA, Gratton E, Sanchez S. Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes. J Phys Chem B 2009; 113:4995-7 Full Text.
  • Chen Y, Veracini L, Benistant C, Jacobson K. The transmembrane protein CBP plays a role in transiently anchoring small clusters of Thy-1, a GPI-anchored protein, to the cytoskeleton. J. Cell. Sci. 2009; 122:3966-72 Full Text | CMC Update Article.
  • Digman MA, Gratton E. Imaging barriers to diffusion by pair correlation functions. Biophys. J. 2009; 97:665-73 Full Text | CMC Update Article.
  • Digman MA, Gratton E. Analysis of diffusion and binding in cells using the RICS approach. Microsc. Res. Tech. 2009; 72:323-32 PubMed.
  • Digman MA, Gratton E. Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy. Wiley Interdiscip Rev Syst Biol Med 2009; 1:273-82 PubMed.
  • Digman MA, Wiseman PW, Choi C, Horwitz AR, Gratton E. Stoichiometry of molecular complexes at adhesions in living cells. Proc. Natl. Acad. Sci. U.S.A. 2009; 106:2170-5 Full Text | CMC Update Article.
  • Digman MA, Wiseman PW, Horwitz AR, Gratton E. Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method. Biophys. J. 2009; 96:707-16 Full Text | CMC Update Article.
  • Huang C, Rajfur Z, Yousefi N, Chen Z, Jacobson K, Ginsberg MH. Talin phosphorylation by Cdk5 regulates Smurf1-mediated talin head ubiquitylation and cell migration. Nat. Cell Biol. 2009; 11:624-30 Full Text | CMC Update Article.
  • Leyman S, Sidani M, Ritsma L, Waterschoot D, Eddy R, Dewitte D, Debeir O, Decaestecker C, Vandekerckhove J, van Rheenen J, Ampe C, Condeelis J, Van Troys M. Unbalancing the phosphatidylinositol-4,5-bisphosphate-cofilin interaction impairs cell steering. Mol. Biol. Cell 2009; 20:4509-23 Full Text | CMC Update Article.
  • Loving G, Imperiali B. Thiol-reactive derivatives of the solvatochromic 4-N,N-dimethylamino-1,8-naphthalimide fluorophore: a highly sensitive toolset for the detection of biomolecular interactions. Bioconjug. Chem. 2009; 20:2133-41 Full Text.
  • McLean MA, Rajfur Z, Chen Z, Humphrey D, Yang B, Sligar SG, Jacobson K. Mechanism of chromophore assisted laser inactivation employing fluorescent proteins. Anal. Chem. 2009; 81:1755-61 Full Text | CMC Update Article.
  • Oser M, Condeelis J. The cofilin activity cycle in lamellipodia and invadopodia. J. Cell. Biochem. 2009; 108:1252-62 PubMed.
  • Oser M, Yamaguchi H, Mader CC, Bravo-Cordero JJ, Arias M, Chen X, Desmarais V, van Rheenen J, Koleske AJ, Condeelis J. Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation. J. Cell Biol. 2009; 186:571-87 Full Text.
  • Rubinstein B, Fournier MF, Jacobson K, Verkhovsky AB, Mogilner A. Actin-myosin viscoelastic flow in the keratocyte lamellipod. Biophys. J. 2009; 97:1853-63 Full Text | CMC Update Article.
  • van Rheenen J, Condeelis J, Glogauer M. A common cofilin activity cycle in invasive tumor cells and inflammatory cells. J. Cell. Sci. 2009; 122:305-11 Full Text | CMC Update Article.
  • Weinreb GE, Kapustina MT, Jacobson K, Elston TC. In silico generation of alternative hypotheses using causal mapping (CMAP). PLoS ONE 2009; 4:e5378 Full Text | CMC Update Article.

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  • Bachir AI, Kolin DL, Heinze KG, Hebert B, Wiseman PW. A guide to accurate measurement of diffusion using fluorescence correlation techniques with blinking quantum dot nanoparticle labels. J Chem Phys 2008; 128:225105 PubMed.
  • Brown CM, Dalal RB, Hebert B, Digman MA, Horwitz AR, Gratton E. Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope. J Microsc 2008; 229:78-91 PubMed | CMC Update Article.
  • Cai X, Lietha D, Ceccarelli DF, Karginov AV, Rajfur Z, Jacobson K, Hahn KM, Eck MJ, Schaller MD. Spatial and temporal regulation of focal adhesion kinase activity in living cells. Mol. Cell. Biol. 2008; 28:201-14 Full Text | CMC Update Article.
  • Choi CK, Vicente-Manzanares M, Zareno J, Whitmore LA, Mogilner A, Horwitz AR. Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. Nat. Cell Biol. 2008; 10:1039-50 Full Text | CMC Update Article.
  • Comeau JW, Kolin DL, Wiseman PW. Accurate measurements of protein interactions in cells via improved spatial image cross-correlation spectroscopy. Mol Biosyst 2008; 4:672-85 PubMed.
  • Dalal RB, Digman MA, Horwitz AF, Vetri V, Gratton E. Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode. Microsc. Res. Tech. 2008; 71:69-81 PubMed | CMC Update Article.
  • Digman MA, Brown CM, Horwitz AR, Mantulin WW, Gratton E. Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy. Biophys. J. 2008; 94:2819-31 Full Text.
  • Digman MA, Caiolfa VR, Zamai M, Gratton E. The phasor approach to fluorescence lifetime imaging analysis. Biophys. J. 2008; 94:L14-6 Full Text | CMC Update Article.
  • Digman MA, Dalal R, Horwitz AF, Gratton E. Mapping the number of molecules and brightness in the laser scanning microscope. Biophys. J. 2008; 94:2320-32 Full Text | CMC Update Article.
  • El-Sibai M, Pertz O, Pang H, Yip SC, Lorenz M, Symons M, Condeelis JS, Hahn KM, Backer JM. RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells. Exp. Cell Res. 2008; 314:1540-52 Full Text.
  • Jacobson K, Rajfur Z, Vitriol E, Hahn K. Chromophore-assisted laser inactivation in cell biology. Trends Cell Biol. 2008; 18:443-50 PubMed | CMC Update Article.
  • Kapustina M, Weinreb GE, Costigliola N, Rajfur Z, Jacobson K, Elston TC. Mechanical and biochemical modeling of cortical oscillations in spreading cells. Biophys. J. 2008; 94:4605-20 Full Text.
  • Kedrin D, Gligorijevic B, Wyckoff J, Verkhusha VV, Condeelis J, Segall JE, van Rheenen J. Intravital imaging of metastatic behavior through a mammary imaging window. Nat. Methods 2008; 5:1019-21 Full Text | CMC Update Article.
  • Macdonald A, Horwitz AR, Lauffenburger DA. Kinetic model for lamellipodal actin-integrin 'clutch' dynamics. Cell Adh Migr 2008; 2:95-105 Full Text | CMC Update Article.
  • Murase S, Cho C, White JM, Horwitz AF. ADAM2 promotes migration of neuroblasts in the rostral migratory stream to the olfactory bulb. Eur. J. Neurosci. 2008; 27:1585-95 Full Text | CMC Update Article.

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  • Aemissegger, A, Carrigan, CN, Imperiali, B. Caged O-phosphorothioyl amino acids as building blocks for Fmoc-based solid phase peptide synthesis. Tetrahedron 2007; 63:6185-90 DOI.
  • Durisic N, Bachir AI, Kolin DL, Hebert B, Lagerholm BC, Grutter P, Wiseman PW. Detection and correction of blinking bias in image correlation transport measurements of quantum dot tagged macromolecules. Biophys. J. 2007; 93:1338-46 Full Text.
  • El-Sibai M, Nalbant P, Pang H, Flinn RJ, Sarmiento C, Macaluso F, Cammer M, Condeelis JS, Hahn KM, Backer JM. Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells. J. Cell. Sci. 2007; 120:3465-74 PubMed | CMC Update Article.
  • Loitto VM, Huang C, Sigal YJ, Jacobson K. Filopodia are induced by aquaporin-9 expression. Exp. Cell Res. 2007; 313:1295-306 PubMed | CMC Update Article.
  • Schober JM, Komarova YA, Chaga OY, Akhmanova A, Borisy GG. Microtubule-targeting-dependent reorganization of filopodia. J. Cell. Sci. 2007; 120:1235-44 PubMed.
  • Sidani M, Wessels D, Mouneimne G, Ghosh M, Goswami S, Sarmiento C, Wang W, Kuhl S, El-Sibai M, Backer JM, Eddy R, Soll D, Condeelis J. Cofilin determines the migration behavior and turning frequency of metastatic cancer cells. J. Cell Biol. 2007; 179:777-91 Full Text | CMC Update Article.
  • van Rheenen J, Song X, van Roosmalen W, Cammer M, Chen X, Desmarais V, Yip SC, Backer JM, Eddy RJ, Condeelis JS. EGF-induced PIP2 hydrolysis releases and activates cofilin locally in carcinoma cells. J. Cell Biol. 2007; 179:1247-59 Full Text | CMC Update Article.
  • Vitriol EA, Uetrecht AC, Shen F, Jacobson K, Bear JE. Enhanced EGFP-chromophore-assisted laser inactivation using deficient cells rescued with functional EGFP-fusion proteins. Proc. Natl. Acad. Sci. U.S.A. 2007; 104:6702-7 Full Text | CMC Update Article.
  • Yam PT, Wilson CA, Ji L, Hebert B, Barnhart EL, Dye NA, Wiseman PW, Danuser G, Theriot JA. Actin-myosin network reorganization breaks symmetry at the cell rear to spontaneously initiate polarized cell motility. J. Cell Biol. 2007; 178:1207-21 Full Text | CMC Update Article.

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  • Brown CM, Hebert B, Kolin DL, Zareno J, Whitmore L, Horwitz AR, Wiseman PW. Probing the integrin-actin linkage using high-resolution protein velocity mapping. J. Cell. Sci. 2006; 119:5204-14 PubMed | CMC Update Article.
  • Kolin DL, Ronis D, Wiseman PW. k-Space image correlation spectroscopy: a method for accurate transport measurements independent of fluorophore photophysics. Biophys. J. 2006; 91:3061-75 Full Text.
  • Lagerholm BC, Averett L, Weinreb GE, Jacobson K, Thompson NL. Analysis method for measuring submicroscopic distances with blinking quantum dots. Biophys. J. 2006; 91:3050-60 Full Text | CMC Update Article.
  • Moissoglu K, Slepchenko BM, Meller N, Horwitz AF, Schwartz MA. In vivo dynamics of Rac-membrane interactions. Mol. Biol. Cell 2006; 17:2770-9 Full Text | CMC Update Article.
  • Prass M, Jacobson K, Mogilner A, Radmacher M. Direct measurement of the lamellipodial protrusive force in a migrating cell. J. Cell Biol. 2006; 174:767-72 Full Text | CMC Update Article.
  • Sakakibara A, Horwitz AF. Mechanism of polarized protrusion formation on neuronal precursors migrating in the developing chicken cerebellum. J. Cell. Sci. 2006; 119:3583-92 PubMed | CMC Update Article.
  • Wang YL, Hahn KM, Murphy RF, Horwitz AF. From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19-21, 2006. J. Cell Biol. 2006; 174:481-4 Full Text | CMC Update Article.
  • Weinreb GE, Elston TC, Jacobson K. Causal mapping as a tool to mechanistically interpret phenomena in cell motility: application to cortical oscillations in spreading cells. Cell Motil. Cytoskeleton 2006; 63:523-32 PubMed | CMC Update Article.

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  • Carbonell WS, Murase S, Horwitz AF, Mandell JW. Migration of perilesional microglia after focal brain injury and modulation by CC chemokine receptor 5: an in situ time-lapse confocal imaging study. J. Neurosci. 2005; 25:7040-7 PubMed.
  • Carbonell WS, Murase SI, Horwitz AF, Mandell JW. Infiltrative microgliosis: activation and long-distance migration of subependymal microglia following periventricular insults. J Neuroinflammation 2005; 2:5 Full Text.
  • Damljanović V, Lagerholm BC, Jacobson K. Bulk and micropatterned conjugation of extracellular matrix proteins to characterized polyacrylamide substrates for cell mechanotransduction assays. BioTechniques 2005; 39:847-51 PubMed.
  • Digman MA, Brown CM, Sengupta P, Wiseman PW, Horwitz AR, Gratton E. Measuring fast dynamics in solutions and cells with a laser scanning microscope. Biophys. J. 2005; 89:1317-27 Full Text | CMC Update Article.
  • Digman MA, Sengupta P, Wiseman PW, Brown CM, Horwitz AR, Gratton E. Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure. Biophys. J. 2005; 88:L33-6 Full Text | CMC Update Article.
  • Hebert B, Costantino S, Wiseman PW. Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells. Biophys. J. 2005; 88:3601-14 Full Text | CMC Update Articles: 1, 2.
  • Humphrey, D, Rajfur, Z, Imperiali, B, Marriott, G, Roy, P, Jacobson, K. Application of Light-directed Activation of Caged Biomolecules and CALI to Problems in Cell Motility.. Living Cell Imaging: A Laboratory Manual 2005; Editors: Goldman and Spector:Cold Spring Harbor Press .
  • Humphrey D, Rajfur Z, Vazquez ME, Scheswohl D, Schaller MD, Jacobson K, Imperiali B. In situ photoactivation of a caged phosphotyrosine peptide derived from focal adhesion kinase temporarily halts lamellar extension of single migrating tumor cells. J. Biol. Chem. 2005; 280:22091-101 PubMed | CMC Update Articles: 1, 2.
  • Kandere-Grzybowska K, Campbell C, Komarova Y, Grzybowski BA, Borisy GG. Molecular dynamics imaging in micropatterned living cells. Nat. Methods 2005; 2:739-41 PubMed | CMC Update Article.
  • Rothman DM, Petersson EJ, Vázquez ME, Brandt GS, Dougherty DA, Imperiali B. Caged phosphoproteins. J. Am. Chem. Soc. 2005; 127:846-7 PubMed | CMC Update Article.
  • Rubinstein, B, Jacobson, K, Mogilner, A. Multiscale Two-Dimensional Modeling of a Motile Simple-Shaped Cell.. SIAM J. MMS, 2005; 3:413-439 Full Text.

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  • Huang C, Borchers CH, Schaller MD, Jacobson K. Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells. J. Cell Biol. 2004; 164:593-602 Full Text.
  • Huang C, Jacobson K, Schaller MD. MAP kinases and cell migration. J. Cell. Sci. 2004; 117:4619-28 PubMed.
  • Murase S, Horwitz AF. Directions in cell migration along the rostral migratory stream: the pathway for migration in the brain. Curr. Top. Dev. Biol. 2004; 61:135-52 PubMed.
  • Nguyen A, Rothman DM, Stehn J, Imperiali B, Yaffe MB. Caged phosphopeptides reveal a temporal role for 14-3-3 in G1 arrest and S-phase checkpoint function. Nat. Biotechnol. 2004; 22:993-1000 PubMed.
  • Pomorski P, Watson JM, Haskill S, Jacobson KA. How adhesion, migration, and cytoplasmic calcium transients influence interleukin-1beta mRNA stabilization in human monocytes. Cell Motil. Cytoskeleton 2004; 57:143-57 PubMed.
  • Roy P, Jacobson K. Overexpression of profilin reduces the migration of invasive breast cancer cells. Cell Motil. Cytoskeleton 2004; 57:84-95 PubMed.
  • Wiseman PW, Brown CM, Webb DJ, Hebert B, Johnson NL, Squier JA, Ellisman MH, Horwitz AF. Spatial mapping of integrin interactions and dynamics during cell migration by image correlation microscopy. J. Cell. Sci. 2004; 117:5521-34 PubMed | CMC Update Articles: 1, 2.

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  • Berland K, Jacobson K, French T, Rajfur Z. Electronic cameras for low-light microscopy. Methods Cell Biol. 2003; 72:103-32 PubMed.
  • Marriott G, Roy P, Jacobson K. Preparation and light-directed activation of caged proteins. Meth. Enzymol. 2003; 360:274-88 PubMed.
  • Rothman DM, Vazquez ME, Vogel EM, Imperiali B. Caged phospho-amino acid building blocks for solid-phase peptide synthesis. J. Org. Chem. 2003; 68:6795-8 PubMed | CMC Update Article.
  • Vázquez ME, Nitz M, Stehn J, Yaffe MB, Imperiali B. Fluorescent caged phosphoserine peptides as probes to investigate phosphorylation-dependent protein associations. J. Am. Chem. Soc. 2003; 125:10150-1 PubMed.
  • Webb DJ, Brown CM, Horwitz AF. Illuminating adhesion complexes in migrating cells: moving toward a bright future. Curr. Opin. Cell Biol. 2003; 15:614-20 PubMed.

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  • Murase S, Horwitz AF. Deleted in colorectal carcinoma and differentially expressed integrins mediate the directional migration of neural precursors in the rostral migratory stream. J. Neurosci. 2002; 22:3568-79 PubMed.
  • Rajfur Z, Roy P, Otey C, Romer L, Jacobson K. Dissecting the link between stress fibres and focal adhesions by CALI with EGFP fusion proteins. Nat. Cell Biol. 2002; 4:286-93 PubMed.
  • Rothman DM, Vázquez ME, Vogel EM, Imperiali B. General method for the synthesis of caged phosphopeptides: tools for the exploration of signal transduction pathways. Org. Lett. 2002; 4:2865-8 PubMed | CMC Update Article.
  • Roy P, Rajfur Z, Pomorski P, Jacobson K. Microscope-based techniques to study cell adhesion and migration. Nat. Cell Biol. 2002; 4:E91-6 PubMed.
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Software

Image Correlation Microscopy

Photomicrograph analysis tools

  • A community resource provided by L. Machesky

    The two applications are ImageJ plugins which are useful for analyzing stacks of photomicrographs taken of cancer cells degrading fluorescently labeled extracellular matrix (ECM). Invadopodia-3 is compiled for use on a PC workstation, while Invadopodia Mac is compiled for use on a Macintosh computer. The short instructions include examples of how to prepare and process photos before using the plugin. The input to the plugin will be two stacks of photos all at the same size and magnification, one with the whole cell visualized using a stain such as fluorescent phalloidin or GFP and the other with matched images of degraded ECM. The output is the total area of degradation. From this output one can measure the average area of degradation per cell or per invadopodium spot. For a recent publication where this plugin has been used to quantify invadopodia degradations, please see:

    1. Li, A., Dawson, J.C., Forero-Vargas, M., Spence, H.J., Yu, X., Konig, I., Anderson, K., and Machesky, L.M. The actin-bundling protein fascin stabilizes actin in invadopodia and potentiates protrusive invasion. 2010 Curr Biol 20, 339-345. PubMed
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