Publications - last updated September, 2010

2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

• Cirit M, Krajcovic M, Choi CK, Welf ES, Horwitz AF, Haugh JM. Stochastic model of integrin-mediated signaling and adhesion dynamics at the leading edges of migrating cells. PLoS Comput. Biol. 2010; 6:e1000688 PubMed | CMC Update Article.
• Costigliola N, Kapustina MT, Weinreb GE, Monteith A, Rajfur Z, Elston TC, Jacobson K. RhoA regulates calcium-independent periodic contractions of the cell cortex. Biophys. J. 2010; 99:1053-63 PubMed | CMC Update Article.
• Jacobson, K. Fluorescence Microscopy. In: Encyclopedia of Life Sciences (ELS). 2010; John Wiley & Sons, Ltd; Chichester: DOI.
• Kapustina M, Vitriol E, Elston TC, Loew LM, Jacobson K. Modeling capping protein FRAP and CALI experiments reveals in vivo regulation of actin dynamics. Cytoskeleton (Hoboken) 2010; 67:519-34 PubMed | CMC Update Article.
• Moens PD, Gratton E, Salvemini IL. Fluorescence correlation spectroscopy, raster image correlation spectroscopy, and number and brightness on a commercial confocal laser scanning microscope with analog detectors (Nikon C1). Microsc Res Tech 2010; PubMed.
• Neumann AK, Jacobson K. A novel pseudopodial component of the dendritic cell anti-fungal response: the fungipod. PLoS Pathog. 2010; 6:e1000760 PubMed | CMC Update Article.
• Ossato G, Digman MA, Aiken C, Lukacsovich T, Marsh JL, Gratton E. A two-step path to inclusion formation of huntingtin peptides revealed by number and brightness analysis. Biophys. J. 2010; 98:3078-85 PubMed | CMC Update Article.

2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

• Caracciolo G, Caminiti R, Digman MA, Gratton E, Sanchez S. Efficient escape from endosomes determines the superior efficiency of multicomponent lipoplexes. J Phys Chem B 2009; 113:4995-7 PubMed.
• Chen Y, Veracini L, Benistant C, Jacobson K. The transmembrane protein CBP plays a role in transiently anchoring small clusters of Thy-1, a GPI-anchored protein, to the cytoskeleton. J. Cell. Sci. 2009; 122:3966-72 PubMed | CMC Update Article.
• Digman MA, Gratton E. Imaging barriers to diffusion by pair correlation functions. Biophys. J. 2009; 97:665-73 PubMed | CMC Update Article.
• Digman MA, Gratton E. Analysis of diffusion and binding in cells using the RICS approach. Microsc. Res. Tech. 2009; 72:323-32 PubMed.
• Digman MA, Wiseman PW, Choi C, Horwitz AR, Gratton E. Stoichiometry of molecular complexes at adhesions in living cells. Proc. Natl. Acad. Sci. U.S.A. 2009; 106:2170-5 PubMed | CMC Update Article.
• Digman MA, Wiseman PW, Horwitz AR, Gratton E. Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method. Biophys. J. 2009; 96:707-16 PubMed | CMC Update Article.
• Huang C, Rajfur Z, Yousefi N, Chen Z, Jacobson K, Ginsberg MH. Talin phosphorylation by Cdk5 regulates Smurf1-mediated talin head ubiquitylation and cell migration. Nat. Cell Biol. 2009; 11:624-30 PubMed | CMC Update Article.
• Leyman S, Sidani M, Ritsma L, Waterschoot D, Eddy R, Dewitte D, Debeir O, Decaestecker C, Vandekerckhove J, van Rheenen J, Ampe C, Condeelis J, Van Troys M. Unbalancing the PI(4,5)P2-Cofilin Interaction Impairs Cell Steering. Mol. Biol. Cell 2009; PubMed | CMC Update Article.
• Loving G, Imperiali B. Thiol-reactive derivatives of the solvatochromic 4-N,N-dimethylamino-1,8-naphthalimide fluorophore: a highly sensitive toolset for the detection of biomolecular interactions. Bioconjug. Chem. 2009; 20:2133-41 PubMed.
• McLean MA, Rajfur Z, Chen Z, Humphrey D, Yang B, Sligar SG, Jacobson K. Mechanism of Chromophore Assisted Laser Inactivation Employing Fluorescent Proteins. Anal. Chem. 2009; PubMed | CMC Update Article.
• Oser M, Condeelis J. The cofilin activity cycle in lamellipodia and invadopodia. J. Cell. Biochem. 2009; PubMed.
• Oser M, Yamaguchi H, Mader CC, Bravo-Cordero JJ, Arias M, Chen X, Desmarais V, van Rheenen J, Koleske AJ, Condeelis J. Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation. J. Cell Biol. 2009; 186:571-87 PubMed.
• van Rheenen J, Condeelis J, Glogauer M. A common cofilin activity cycle in invasive tumor cells and inflammatory cells. J. Cell. Sci. 2009; 122:305-11 PubMed | CMC Update Article.
• Weinreb GE, Kapustina MT, Jacobson K, Elston TC. In silico generation of alternative hypotheses using causal mapping (CMAP). PLoS ONE 2009; 4:e5378 PubMed | CMC Update Article.

2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

• Brown CM, Dalal RB, Hebert B, Digman MA, Horwitz AR, Gratton E. Raster image correlation spectroscopy (RICS) for measuring fast protein dynamics and concentrations with a commercial laser scanning confocal microscope. J Microsc 2008; 229:78-91 PubMed | CMC Update Article.
• Cai X, Lietha D, Ceccarelli DF, Karginov AV, Rajfur Z, Jacobson K, Hahn KM, Eck MJ, Schaller MD. Spatial and temporal regulation of focal adhesion kinase activity in living cells. Mol. Cell. Biol. 2008; 28:201-14 PubMed | CMC Update Article.
• Choi CK, Vicente-Manzanares M, Zareno J, Whitmore LA, Mogilner A, Horwitz AR. Actin and alpha-actinin orchestrate the assembly and maturation of nascent adhesions in a myosin II motor-independent manner. Nat. Cell Biol. 2008; PubMed | CMC Update Article.
• Comeau JW, Kolin DL, Wiseman PW. Accurate measurements of protein interactions in cells via improved spatial image cross-correlation spectroscopy. Mol Biosyst 2008; 4:672-85 PubMed.
• Dalal RB, Digman MA, Horwitz AF, Vetri V, Gratton E. Determination of particle number and brightness using a laser scanning confocal microscope operating in the analog mode. Microsc. Res. Tech. 2008; 71:69-81 PubMed | CMC Update Article.
• Digman MA, Brown CM, Horwitz AR, Mantulin WW, Gratton E. Paxillin dynamics measured during adhesion assembly and disassembly by correlation spectroscopy. Biophys. J. 2008; 94:2819-31 PubMed.
• Digman MA, Caiolfa VR, Zamai M, Gratton E. The phasor approach to fluorescence lifetime imaging analysis. Biophys. J. 2008; 94:L14-6 PubMed | CMC Update Article.
• Digman MA, Dalal R, Horwitz AF, Gratton E. Mapping the number of molecules and brightness in the laser scanning microscope. Biophys. J. 2008; 94:2320-32 PubMed | CMC Update Article.
• Jacobson K, Rajfur Z, Vitriol E, Hahn K. Chromophore-assisted laser inactivation in cell biology. Trends Cell Biol. 2008; 18:443-50 PubMed | CMC Update Article.
• Kapustina M, Weinreb GE, Costigliola N, Rajfur Z, Jacobson K, Elston TC. Mechanical and biochemical modeling of cortical oscillations in spreading cells. Biophys. J. 2008; 94:4605-20 PubMed.
• Kedrin D, Gligorijevic B, Wyckoff J, Verkhusha VV, Condeelis J, Segall JE, van Rheenen J. Intravital imaging of metastatic behavior through a mammary imaging window. Nat. Methods 2008; 5:1019-21 PubMed | CMC Update Article.
• Macdonald A, Horwitz AR, Lauffenburger DA. Kinetic model for lamellipodal actin-integrin 'clutch' dynamics. Cell Adh Migr 2008; 2:95-105 PubMed.
• Murase S, Cho C, White JM, Horwitz AF. ADAM2 promotes migration of neuroblasts in the rostral migratory stream to the olfactory bulb. Eur. J. Neurosci. 2008; 27:1585-95 PubMed | CMC Update Article.

2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

• Aemissegger, A, Carrigan, CN, Imperiali, B. Caged O-phosphorothioyl amino acids as building blocks for Fmoc-based solid phase peptide synthesis. Tetrahedron 2007; 63:6185-90 DOI.
• Durisic N, Bachir AI, Kolin DL, Hebert B, Lagerholm BC, Grutter P, Wiseman PW. Detection and correction of blinking bias in image correlation transport measurements of quantum dot tagged macromolecules. Biophys. J. 2007; 93:1338-46 PubMed.
• Loitto VM, Huang C, Sigal YJ, Jacobson K. Filopodia are induced by aquaporin-9 expression. Exp. Cell Res. 2007; 313:1295-306 PubMed | CMC Update Article.
• Schober JM, Komarova YA, Chaga OY, Akhmanova A, Borisy GG. Microtubule-targeting-dependent reorganization of filopodia. J. Cell. Sci. 2007; 120:1235-44 PubMed.
• Sidani M, Wessels D, Mouneimne G, Ghosh M, Goswami S, Sarmiento C, Wang W, Kuhl S, El-Sibai M, Backer JM, Eddy R, Soll D, Condeelis J. Cofilin determines the migration behavior and turning frequency of metastatic cancer cells. J. Cell Biol. 2007; 179:777-91 PubMed | CMC Update Article.
• van Rheenen J, Song X, van Roosmalen W, Cammer M, Chen X, Desmarais V, Yip SC, Backer JM, Eddy RJ, Condeelis JS. EGF-induced PIP2 hydrolysis releases and activates cofilin locally in carcinoma cells. J. Cell Biol. 2007; 179:1247-59 PubMed | CMC Update Article.
• Vitriol EA, Uetrecht AC, Shen F, Jacobson K, Bear JE. Enhanced EGFP-chromophore-assisted laser inactivation using deficient cells rescued with functional EGFP-fusion proteins. Proc. Natl. Acad. Sci. U.S.A. 2007; 104:6702-7 PubMed | CMC Update Article.

2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

• Brown CM, Hebert B, Kolin DL, Zareno J, Whitmore L, Horwitz AR, Wiseman PW. Probing the integrin-actin linkage using high-resolution protein velocity mapping. J. Cell. Sci. 2006; 119:5204-14 PubMed | CMC Update Article.
• Kolin DL, Ronis D, Wiseman PW. k-Space image correlation spectroscopy: a method for accurate transport measurements independent of fluorophore photophysics. Biophys. J. 2006; 91:3061-75 PubMed.
• Lagerholm BC, Averett L, Weinreb GE, Jacobson K, Thompson NL. Analysis method for measuring submicroscopic distances with blinking quantum dots. Biophys. J. 2006; 91:3050-60 PubMed | CMC Update Article.
• Sakakibara A, Horwitz AF. Mechanism of polarized protrusion formation on neuronal precursors migrating in the developing chicken cerebellum. J. Cell. Sci. 2006; 119:3583-92 PubMed | CMC Update Article.
• Wang YL, Hahn KM, Murphy RF, Horwitz AF. From imaging to understanding: Frontiers in Live Cell Imaging, Bethesda, MD, April 19-21, 2006. J. Cell Biol. 2006; 174:481-4 PubMed | CMC Update Article.
• Weinreb GE, Elston TC, Jacobson K. Causal mapping as a tool to mechanistically interpret phenomena in cell motility: application to cortical oscillations in spreading cells. Cell Motil. Cytoskeleton 2006; 63:523-32 PubMed | CMC Update Article.

2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

• Carbonell WS, Murase S, Horwitz AF, Mandell JW. Migration of perilesional microglia after focal brain injury and modulation by CC chemokine receptor 5: an in situ time-lapse confocal imaging study. J. Neurosci. 2005; 25:7040-7 PubMed.
• Carbonell WS, Murase SI, Horwitz AF, Mandell JW. Infiltrative microgliosis: activation and long-distance migration of subependymal microglia following periventricular insults. J Neuroinflammation 2005; 2:5 PubMed.
• Damljanović V, Lagerholm BC, Jacobson K. Bulk and micropatterned conjugation of extracellular matrix proteins to characterized polyacrylamide substrates for cell mechanotransduction assays. BioTechniques 2005; 39:847-51 PubMed.
• Digman MA, Brown CM, Sengupta P, Wiseman PW, Horwitz AR, Gratton E. Measuring fast dynamics in solutions and cells with a laser scanning microscope. Biophys. J. 2005; 89:1317-27 PubMed | CMC Update Article.
• Digman MA, Sengupta P, Wiseman PW, Brown CM, Horwitz AR, Gratton E. Fluctuation correlation spectroscopy with a laser-scanning microscope: exploiting the hidden time structure. Biophys. J. 2005; 88:L33-6 PubMed | CMC Update Article.
• Hebert B, Costantino S, Wiseman PW. Spatiotemporal image correlation spectroscopy (STICS) theory, verification, and application to protein velocity mapping in living CHO cells. Biophys. J. 2005; 88:3601-14 PubMed | CMC Update Article.
• Humphrey, D, Rajfur, Z, Imperiali, B, Marriott, G, Roy, P, Jacobson, K. Application of Light-directed Activation of Caged Biomolecules and CALI to Problems in Cell Motility.. Living Cell Imaging: A Laboratory Manual 2005; Editors: Goldman and Spector:Cold Spring Harbor Press .
• Humphrey D, Rajfur Z, Vazquez ME, Scheswohl D, Schaller MD, Jacobson K, Imperiali B. In situ photoactivation of a caged phosphotyrosine peptide derived from focal adhesion kinase temporarily halts lamellar extension of single migrating tumor cells. J. Biol. Chem. 2005; 280:22091-101 PubMed.
• Kandere-Grzybowska K, Campbell C, Komarova Y, Grzybowski BA, Borisy GG. Molecular dynamics imaging in micropatterned living cells. Nat. Methods 2005; 2:739-41 PubMed | CMC Update Article.
• Rothman DM, Petersson EJ, Vázquez ME, Brandt GS, Dougherty DA, Imperiali B. Caged phosphoproteins. J. Am. Chem. Soc. 2005; 127:846-7 PubMed | CMC Update Article.
• Rubinstein, B, Jacobson, K, Mogilner, A. Multiscale Two-Dimensional Modeling of a Motile Simple-Shaped Cell.. SIAM J. MMS, 2005; 3:413-439 .
• Rubinstein, B, Jacobson, K, Mogilner, A. Multiscale Two-Dimensional Modeling of a Motile Simple-Shaped Cell.. SIAM J. MMS, 2005; 3:413-439 PubMed.

2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

• Huang C, Borchers CH, Schaller MD, Jacobson K. Phosphorylation of paxillin by p38MAPK is involved in the neurite extension of PC-12 cells. J. Cell Biol. 2004; 164:593-602 PubMed.
• Huang C, Jacobson K, Schaller MD. MAP kinases and cell migration. J. Cell. Sci. 2004; 117:4619-28 PubMed.
• Murase S, Horwitz AF. Directions in cell migration along the rostral migratory stream: the pathway for migration in the brain. Curr. Top. Dev. Biol. 2004; 61:135-52 PubMed.
• Nguyen A, Rothman DM, Stehn J, Imperiali B, Yaffe MB. Caged phosphopeptides reveal a temporal role for 14-3-3 in G1 arrest and S-phase checkpoint function. Nat. Biotechnol. 2004; 22:993-1000 PubMed.
• Pomorski P, Watson JM, Haskill S, Jacobson KA. How adhesion, migration, and cytoplasmic calcium transients influence interleukin-1beta mRNA stabilization in human monocytes. Cell Motil. Cytoskeleton 2004; 57:143-57 PubMed.
• Roy P, Jacobson K. Overexpression of profilin reduces the migration of invasive breast cancer cells. Cell Motil. Cytoskeleton 2004; 57:84-95 PubMed.
• Wiseman PW, Brown CM, Webb DJ, Hebert B, Johnson NL, Squier JA, Ellisman MH, Horwitz AF. Spatial mapping of integrin interactions and dynamics during cell migration by image correlation microscopy. J. Cell. Sci. 2004; 117:5521-34 PubMed.

2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

• Berland K, Jacobson K, French T, Rajfur Z. Electronic cameras for low-light microscopy. Methods Cell Biol. 2003; 72:103-32 PubMed.
• Marriott G, Roy P, Jacobson K. Preparation and light-directed activation of caged proteins. Meth. Enzymol. 2003; 360:274-88 PubMed.
• Rothman DM, Vazquez ME, Vogel EM, Imperiali B. Caged phospho-amino acid building blocks for solid-phase peptide synthesis. J. Org. Chem. 2003; 68:6795-8 PubMed | CMC Update Article.
• Vázquez ME, Nitz M, Stehn J, Yaffe MB, Imperiali B. Fluorescent caged phosphoserine peptides as probes to investigate phosphorylation-dependent protein associations. J. Am. Chem. Soc. 2003; 125:10150-1 PubMed.
• Webb DJ, Brown CM, Horwitz AF. Illuminating adhesion complexes in migrating cells: moving toward a bright future. Curr. Opin. Cell Biol. 2003; 15:614-20 PubMed.

2010 | 2009 | 2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

• Murase S, Horwitz AF. Deleted in colorectal carcinoma and differentially expressed integrins mediate the directional migration of neural precursors in the rostral migratory stream. J. Neurosci. 2002; 22:3568-79 PubMed.
• Rajfur Z, Roy P, Otey C, Romer L, Jacobson K. Dissecting the link between stress fibres and focal adhesions by CALI with EGFP fusion proteins. Nat. Cell Biol. 2002; 4:286-93 PubMed.
• Rothman DM, Vázquez ME, Vogel EM, Imperiali B. General method for the synthesis of caged phosphopeptides: tools for the exploration of signal transduction pathways. Org. Lett. 2002; 4:2865-8 PubMed | CMC Update Article.
• Roy P, Rajfur Z, Pomorski P, Jacobson K. Microscope-based techniques to study cell adhesion and migration. Nat. Cell Biol. 2002; 4:E91-6 PubMed.