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Genetic Activities/Screening Approaches

Drosophila Morphology Genes

This website contains the primary data of published work. Please cite any reference to this work as: D’Ambrosio, M.V. and R. Vale. “A whole genome RNAi screen of Drosophila S2 cell spreading performed using automated computational image analysis.” Journal of Cell Biology (2010) 191:471-478 PubMed.

See footnotes below for experiment description and category definitions.
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Morphology quintiles: lowestlowmiddlehighhighest
  GeneNameCell Count/
Normalization
Stellate11
NumCorners
Stellate22
Binsum6
Non-Spreading3
1 140up
Entrez: CG9852
Flybase: FBgn0010340
upstream of RpII1404.750
0.830
0.9160.96Actin: 0.836
Area: 1.091
Ratio: 0.766
2 14-3-3epsilon
Entrez: CG31196
Flybase: FBgn0020238
14-3-3epsilon
Aliases: Suppressor of Ras85D 3-9; 14-3-3e
5.625
1.112
0.8261.035Actin: 1.275
Area: 0.946
Ratio: 1.348
3 14-3-3zeta
Entrez: CG17870
Flybase: FBgn0004907
14-3-3zeta
Aliases: leonardo; Leonardo-13-3-3; leonardo 14-3-3; Complementation group K; D14 3 3 protein; Leonardo
4.886
0.776
0.8630.955Actin: 1.192
Area: 0.949
Ratio: 1.256
4 18w
Entrez: CG8896
Flybase: FBgn0004364
18 wheeler
Aliases: 18-wheeler; 18-Wheeler; wheeler; Toll like receptor; toll receptor; Toll-2; 18Wheeler; 18wheeler
8.375
1.415
0.9031.065Actin: 1.035
Area: 0.974
Ratio: 1.063
5 26-29-p
Entrez: CG8947
Flybase: FBgn0250848
26-29kD-proteinase
Aliases: 26,29kDa proteinase; 26-29kDa proteinase; cathepsin K; anon-SAGE:Wang-123; 26-29 kD-proteinase
5.000
1.026
1.2140.954Actin: 0.805
Area: 1.186
Ratio: 0.679
6 312
Entrez: CG9166
Flybase: FBgn0029514
3127.375
1.262
1.0171.041Actin: 1.042
Area: 0.931
Ratio: 1.119
7 4EHP
Entrez: CG33100
Flybase: FBgn0053100
4EHP4.800
0.991
0.8550.974Actin: 0.954
Area: 0.917
Ratio: 1.040
8 5-HT1A
Entrez: CG16720
Flybase: FBgn0004168
Serotonin receptor 1A
Aliases: serotonin-receptor-2A; Serotonin receptor 2A
6.563
0.762
1.2841.108Actin: 1.347
Area: 1.025
Ratio: 1.314
9 5-HT2
Entrez: CG1056
Flybase: FBgn0087012
Serotonin receptor 2
Aliases: 5-HT[[2]]Dro receptor; lethal (3) 82CDb
5.333
0.969
1.030.929Actin: 0.936
Area: 1.139
Ratio: 0.822
10 5-HT7
Entrez: CG12073
Flybase: FBgn0004573
Serotonin receptor 7
Aliases: Serotonin receptor; Serotonin receptor 1
5.789
0.949
1.1030.949Actin: 0.847
Area: 1.083
Ratio: 0.782
11 5PtaseI
Entrez: CG31110
Flybase: FBgn0259178
5PtaseI
Aliases: CG31110; CG42283
4.727
0.976
1.2891.02Actin: 1.014
Area: 0.997
Ratio: 1.017
12 5PtaseI
Entrez: CG31107
Flybase: FBgn0259178
5PtaseI
Aliases: CG31110; CG42283
5.000
1.062
0.7430.914Actin: 1.041
Area: 1.107
Ratio: 0.940
13 7B2
Entrez: CG1168
Flybase: FBgn0041707
7B25.429
1.081
1.0111.033Actin: 0.945
Area: 1.004
Ratio: 0.941
14 825-Oak
Entrez: CG32208
Flybase: FBgn0052208
825-Oak2.600
0.630
1.1881.015Actin: 0.705
Area: 1.005
Ratio: 0.701
15 a
Entrez: CG6741
Flybase: FBgn0000008
arc
Aliases: broad angular
5.762
1.228
0.670.833Actin: 1.136
Area: 1.045
Ratio: 1.087
16 a
Entrez: CG6741
Flybase: FBgn0000008
arc
Aliases: broad angular
6.813
0.808
0.8910.913Actin: 1.277
Area: 1.051
Ratio: 1.215
17 a10
Entrez: CG6642
Flybase: FBgn0011293
antennal protein 10
Aliases: Pherokine1; Pherokine 1
4.500
0.936
0.9320.986Actin: 0.839
Area: 0.937
Ratio: 0.895
18 A16
Entrez: CG9933
Flybase: FBgn0028965
A165.278
1.173
0.80.947Actin: 1.042
Area: 1.128
Ratio: 0.924
19 a5
Entrez: CG5430
Flybase: FBgn0011294
antennal protein 57.063
1.069
1.0590.991Actin: 0.94
Area: 1.057
Ratio: 0.889
20 Aac11
Entrez: CG6582
Flybase: FBgn0027885
Aac11
Aliases: apoptosis inhibitor-5; antiapoptosis clone-11
6.333
1.140
0.7270.896Actin: 0.991
Area: 1.101
Ratio: 0.900
Morphology quintiles: lowestlowmiddlehighhighest

Notes:

(1) Stellate1 is the normalized number of corners detected per cell. A corner is a region of high curvature, as assessed by an adaptive method.

(2) Stellate2 is the the area under the curve of a region of a Fourier decomposition of cell protrusion at the membrane. The particular region was selected as being elevated in stellate cells.

(3) Non-spreading is defined as having a high actin/area ratio. This usually denotes cells that have a normal amount of actin staining, but smaller than average area.

The matlab script used to reduce the data is available here.