Genetic Approaches

Last Updated May 2007

C. elegans gonad formation - Primary RNAi screen results

Feeding-mediated RNAi of C. elegans was used to screen for genes that are required for normal gonad formation and distal tip cell migration. The first step in our screen used low-magnification microscopy to identify animals with clear patches, which result from displacement of the intestine due to inappropriate turns of the gonad arms or gonad distension (see Cram et al. (2003) J. Cell Sci. 116: 3871-3878). This screen of 16,758 bacterial strains identified only 3% or 503 strains with some clear patches. Each of the 503 genes was further tested by DIC microscopy in the secondary RNAi screen. Gonad defects were confirmed for 99 of these genes. Supplementary Table 2 lists functional information and homologs for the 99 genes. Supplementary Table 3 gives details of the distal tip cell migration phenotype, statistical analysis, and available expression information for each gene.

Data from:
Cram et al. (2006) "A systematic RNA interference screen reveals a cell migration gene network in C. elegans" J. Cell. Sci. 119:4811 PubMed

Supplementary Table 2: Functional information and homologs. Published version

Supplementary Table 3: DTC migration data, statistical analysis, and phenotypic categorization Published version