| Drosophila Migration Gene Screens |

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Genes encoding cytoskeleton-associated proteins enriched in migratory cells

Gene Symbol	Protein Name	Flybase link	Fold change	Proposed Function	Nearest mammalian homolog	Unigene (human homolog)	Conserved protein domains
Act5C	Actin 5C	FBgn0000042	1.31	Actin subunit	Gamma actin	514581	Actin
Act79B	Actin79B	FBgn0000045	1.48	Actin subunit	Beta actin	520640	Actin
Act42A	Actin 42A	FBgn0000043	1.46	Actin subunit	Gamma actin	514581	Actin
Arpc3A	Actin related protein C3a	FBgn0038369	1.28	Actin filament nucleation and branching	Arp3	490655	P21 arc
Arp66B	Actin related protein	FBgn0011744	1.27	Actin filament nucleation and branching	Arp3	490655	actin
Arp87C	Actin related protein	FBgn0011745	1.08	Actin filament nucleation and branching	Arp1	153961	actin
cib	Ciboulot	FBgn0026084	1.97	Actin monomer binding	Thymosin beta4	522584	THY
cpb	Capping protein beta	FBgn0011570	1.41	Actin binding and capping	Capping protein beta	432760	F_actin_cap_B
CG10540	Capping protein alpha	FBgn0034577	2.24	Barbed end actin capping	Capping protein alpha	514934	F-actin_cap_A
CG5869	None	FBgn0028894	1.27	Actin depolymerization	GMFB	151413	ADF
CG10083	None	FBgn0036372	1.62	Competes with tropomyosin for F-actin binding	Drebrin-like	436500	ADF, SH3
Tm1	Tropomyosin 1	FBgn0003721	1.66	F-actin binding, promotes long, parallel filament bundles	Tropomyosin	133892	Tropomyosin
CG11605	jitterbug	FBgn0028371	2.03	F-actin crosslinking	Filamin A	195464	Filamin type Ig
sn	Singed	FBgn0003447	1.78	F-actin crosslinking/bundling	Fascin	118400	Fascin
Cortactin	Cortactin	FBgn0025865	1.75	Cortical actin binding	Cortactin	567262	SH3
beta_Spec	beta-Spectrin	FBgn0003471	1.58	Actin binding, phospholipid binding Membrane skeleton	Spectrin beta 2	26915	CH, PH, Spectrin repeats
Actn	alpha-actinin	FBgn0000667	1.38	F-actin crosslinking Links talin and F-actin	Actinin alpha 1	509765	CH, EF hand, Spectrin repeats
shot	Short stop	FBgn0013733	1.45	MT and actin cross-linking protein	Dystonin 1eA	485616	Spectrin repeats, EF hand, CH
Chd64	CHD64	FBgn0035499	1.69	Actin binding	Calponin	465929	CH
CG3960	none	FBgn0029876	1.5	Actin binding	Smoothelin	149098	CH
CG31352	none	FBgn0051352	1.45	Actin binding	actin binding LIM protein 1	438236	4X Lim,Villin
rhea	Rhea	FBgn0020254	1.78	Links membrane receptors to the F-actin cytoskeleton	Talin	511686	Band 4.1, FERM-C, PTB, ILWEQ
Ptpmeg	Protein tyrosine phosphatase MEG1	FBgn0035133	1.51	Cytoskeleton associated phosphatase	PtpMEG1	469809	Band 4.1, FERM-C, SH3, PTP
CAP	Rexin	FBgn0033504	1.48	Adaptor	Hypothetical protein	38621	SH3
ck	Crinkled	FBgn0000317	1.74	Plus end directed actin motor	Myosin VIIa	370421	MyosinVII motor
didum	Dilute class myosin	FBgn0015933	1.73	Plus end directed actin motor	Myosin V	21213	Myosin V motor
zip	Zipper	FBgn0005634	1.95	Plus end directed actin motor	Non-muscle myosin II	16355	Myosin II motor
Mhc	Myosin heavy chain	FBgn0002741	1.78	Plus end directed actin motor	Non-muscle myosin heavy chain	278432	MYSc, Myosin_tail_1
Mlc-c	Myosin light chain cytoplasmic	FBgn0004687	1.66	Calcium and myosin heavy chain binding	Non-muscle myosin light chain	505705	EF-Hands
jar	Jaguar	FBgn0011225	1.38	Pointed end directed actin motor	Myosin VI	149387	SH3-like, myosin VI motor
AnnIX	Annexin IX	FBgn0000083	1.77	Membrane organization	Annexin A7	386434	Annexin repeats
AnnX	Annexin X	FBgn0000084	1.31	Membrane fusion and actin binding	Annexin A7	386434	Annexin X
Sep2	Septin2	FBgn0014029	1.42	Cleavage furrow	Septin 11	128199	GTPase
Sep1	Septin-1	FBgn0011710	2.13	Cleavage furrow	Septin 2	335057	GTPase
l(2)gl	Lethal(2)giant larvae	FBgn0002121	2.26	Tumor suppressor, cell polarity	L(2)gl homolog	513983	WD40 repeat
mys	Myospheroid	FBgn0004657	1.17	Cell adhesion	Integrin beta-1	429052	integrin
alpha-Cat	Alpha catenin	FBgn0010215	1.59	Cell adhesion/ actin binding	Alpha catenin	445981	Vinculin family
arm	Armadillo	FBgn0000117	1.41	Adaptor / cell adhesion / transcription	Beta-catenin	476018	Arm repeats
Ras85D	Ras oncogene at 85D	FBgn0003205	1.71	Small GTPase, cell proliferation	c-K-Ras	505033	GTPase
Rab11	Rab protein 11	FBgn0015790	1.88	Small GTPase, slow vesicle recycling	Rab11	321541	GTPase
Synd	Syndapin	FBgn0053094	1.83	Links vesicle trafficking and actin dynamics	Syndapin/Pacsin	162877	Fes/cip4, SH3
msps	Mini spindles	FBgn0027948	1.23	Microtubule associated protein	Riken	201253	HEAT
CG11242	none	FBgn0034451	1.28	Cytoskeleton-associated / tubulin chaperone	CAP1	31053	CAP-GLY
Dhc64C	Dynein heavy chain at 64C	FBgn0010349	1.55	Minus end directed microtubule motor	DNCH1	7720	Dynein_heavy, AAA ATPase
BicD	Bicaudal D	FBgn0000183	1.50	MT and vesicle binding	BicD2	436939	Smc

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The genes in the table above were assembled by searching the Affymetrix-assigned GO terms (biological process, cellular component and molecular function) associated with each of the migratory cell enriched genes for the terms "cytoskeleton" or "actin". This list is not identical to the list of genes associated with the cytoskeleton in the GO-Slim function of Genespring (See Experimental procedures). Abbreviations: ADF-actin depolymerizing factor domain, CH-Calponin homology, PH-Plextrin homology, SH3- src homology 3, PTP-protein tyrosine phosphatase, CAP-cytoskeleton associated protein, MYSc-Myosin motor domain.

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Migratory cell enriched genes encoding proteins associated with vesicle traffic

Gene symbol	Protein name	Flybase link	Cellular component	Fold change_	Proposed function	Nearest mammalian homolog	Unigene (human homolog)
Clc	Clathrin light chain	FBgn0024814	E	1.41	Clathrin coated vesicle formation	Clathrin light chain	522114
Chc	Clathrin heavy chain	FBgn0000319	E	1.27	Clathrin vesicle coat	Clathrin heavy chain	491351
CG31170	none	FBgn0042645	G	2.55	Vesicle budding, Clathrin interacting	Enthoprotin/Clint/ epsinR	132853
lqf	Liquid facets	FBgn0028582	V/ER/G	1.27	endocytic adaptor	Epsin1	279953
Rab5	Rab-protein 5	FBgn0014010	V/E	1.17	Endocytic vesicle transport	Rab5 GTPase	475663
Rab6	Rab-protein 6	FBgn0015797	none	1.34	Recruits dynein/dynactin to vesicles	RAB6 GTPase	503222
Rab11	Rab-protein 11	FBgn0015790	E	1.88	Vesicle trafficking	Rab11A	321541
Gdi	GDP dissociation inhibitor	FBgn0004868	V/ER/G	1.48	Rab GTPase chaperone	Rab GDI Vesicle transport	74576
CG1418	none	FBgn0033468	none	1.51	intracellular protein transport	Rab acceptor 1	11417
Arf51F	ADP ribosylation factor 51F	FBgn0013750	V/G	1.68	Coated vesicle assembly	Arf6	525330
Arf79F	Arf79F	FBgn0010348	V/G	1.70	Coated vesicle assembly	Arf1	286221
siz	schizo	FBgn0026179	none	1.27	ARF6 exchange factor	IQSEC1	475506
a-Cop	alpha-Coatomer protein	FBgn0025725	V/G	1.57	Vesicle transport	Coatomer subunit alpha	162121
b-Cop	beta-Coatomer protein	FBgn0008635	V/G	1.53	Vesicle transport	Coatomer subunit beta	339278
g-Cop	gamma-_Coatomer protein	FBgn0028968	V/G	1.20	Vesicle transport	Coatomer subunit gamma	518250
b'-Cop	beta'-Coatomer protein	FBgn0025724	V/G	1.38	Vesicle transport	Coatomer subunit beta 2 (COPB2)	75724
z-Cop	none	FBgn0040512	V	1.21	Vesicle transport	Coatomer subunit zeta 1	505652
CG11785	none	FBgn0045866	V/ER/G	1.50	ER to golgi transport	Trafficking protein	74137
CG3564	none	FBgn0029709	V/G	1.44	COPI Vesicle transport	Coated vesicle membrane protein	75914
CG1472	none	FBgn0033460	V/ER/G	1.51	ER-Golgi transport/ COPII coated vesicles	Sec24 gene family	292472
CG7011	none	FBgn0036489	V	1.41	ER-Golgi transport/ COPII coated vesicles	SDBCA84	472558
CG7809	none	FBgn0036919	G	1.18	Secretory protein transport and golgi structure	Golgi reassembly stacking protein 2	431317
pip	Pipe	FBgn0003089	G	1.59	Heparan sulfate 2-O-sulfo-transferase	Chondroitin sulfate sulfotransferase	309250
Csat	none	FBgn0024994	G	1.78	Transports sugar nucleotides from cytoplasm to golgi	UDP-galactose transporter	21899
fng	Fringe	FBgn0011591	G/ER	1.40	Acetylglucosaminyltransferase	Fringe homolog	569701
CG12139	none	FBgn0030130	G		Receptor	GP330/LDLR2	470538
CG1275	none	FBgn0035321	G	1.43	oxidoreductase	Cytochrome B reductase	221941
Synd	Syndapin	FBgn0053094	V/ER/G	1.83	Binds dynamin and N-WASP	Syndapin/Pacsin	162877
AnnX	Annexin X	FBgn0000084	none	1.31	Calcium-dependent membrane fusion and actin binding	Annexin A7	386434
Crc	Calreticulin	FBgn0005585	ER	1.26	Folding of newly synthesized (glyco) proteins	Calreticulin	515162
Rtnl1	none	FBgn0053113	ER	1.08	Intracellular protein transport	Reticulon 1	368626
CG5885	none	FBgn0025700	ER	1.36	Intracellular protein transport	signal sequence receptor, gamma	518346
CG8552	none	FBgn0031990	none	1.54	Intracellular protein transport	DDHD domain	434966
Dhc64C	Dynein heavy chain at 64C	FBgn0010349	none	1.55	Minus end directed microtubule motor	DNCH1	7720
jar	Jaguar	FBgn0011225	V/ER/C	1.38	Endocytic vesicle transport	Myosin VI	149387
ck	Crinkled	FBgn0000317	none	1.74	Plus end directed actin motor	Myosin VIIa	370421
didum	dilute class myosin	FBgn0015933	none	1.73	Intracellular protein transport	Myosin VA	21213
CG8776	none	FBgn0033764	V	1.67	Electron transport	CYBASC3	22546
Cirl	CIRL	FBgn0033313	V/ER/G	1.83	G protein coupled receptor	Latrophilin 3	411097
Nrx-IV	Neurexin IV	FBgn0013997	V/ER/G	2.22	Septate junction protein	Contactin associated protein-like 2	567443
l(2)gl	Lethal (2) giant larvae	FBgn0002121	V/ER/G	2.26	Asymmetric protein localization	Lethal giant larvae homolog	513983
Rala	Ras-related protein	FBgn0015286	none	1.72	Ras effector	v-ral	6906
Ras85D	Ras oncogene at 85D	FBgn0003205	none	1.71	Small GTPase, cell proliferation	c-K-Ras	505033
Cnx99A	Calnexin 99A	FBgn0015622	ER	1.40	Folding of newly synthesized (glyco)proteins	Calnexin	529890
PI3K 68D	Phosphotidylinositol 3 kinase 68D	FBgn0015278	PI3 kinase complex	1.29	Phosphoinositide phosphorylation	PIK3C2A	175343
rhea	Rhea	FBgn0020254	none	1.78	Links membrane receptors to the F-actin cytoskeleton	Talin	511686

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This table above was assembled by searching all of the Affymetrix-assigned Go terms (biological process, cellular component and molecular function) associated with each of the migratory cell enriched genes for the terms "vesicle", "endocytosis", or "exocytosis". Abbreviations: V-cytoplasmic vesicle, ER-endoplasmic reticulum, G-Golgi apparatus, E: endosome, CIRL-Calcium independent receptor for latrotoxin, COP-Coatomer subunit, CYBASC3-Cytochrome b, ascorbate dependent 3, SDBCA84- serologically defined breast cancer antigen 84, DNCH1-Dynein, cytoplasmic, heavy chain 1.

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