Cell Migration Gateway Logo

Discovery - Activities

Objectives

  • to use genetic and expression strategies in genetically tractable organisms to identify migration related genes
  • to use expression cloning strategies to identify proteins involved in cell migration
  • to screen chemical libraries for reagents that perturb migration, adhesion or cytoskeletal organization

Collaboration and Release Policy

Genetic Approaches | Gene Screens | Chemical Screens

Genetic Approaches

Organism Approach Status / Publication Contact (Collaborators)
D. melanogaster identification of genes upregulated in migrating cells Completed - Data available Montell
D. melanogaster identification of genes encoding cytoskeleton-associated proteins enriched in migratory cells Completed - Data available Montell
D. melanogaster identification of migratory cell enriched genes encoding proteins associated with vesicle traffic Completed - Data available Montell
C. elegans RNAi screen for DTC migration defects Completed - Data available Schwarzbauer
D. melanogaster Collection of published cell migration genes in Drosophila (multiple cell types) Primordial germ cell genes, Border cell genes Montell
D. melanogaster identification of downstream targets of migration-related transcription factors Data available Montell
D. melanogaster matching border cell genes with migration defective mutants In progress Montell
D. melanogaster identification of genes that alter S2 morphology using large scale RNAi screening and high resolution/high-throughput microscopy In progress Vale
D. melanogaster characterization of the proteins identified in the high throughput screen In progress Vale
C. elegans Determine cell autonomy of 99 DTC migration genes Proposed Schwarzbauer
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Gene Screens

Phenotype Screen Target Cell Library (cDNA/siRNA) Status / Publication Contact (Collaborators)
Migration Transwell assay for induction of migration MCF-10A expressing activated ErbB2 BC-1000 cDNA Completed - Data available Brugge (LaBaer)
Migration Transwell assay for induction of migration MCF-10A MCF-7 cDNA Completed - Data available Brugge
Migration Transwell assay for induction of migration MCF-10A expressing CSF-1R MCF-7 cDNA Completed - Data available Brugge
Migration Transwell assay for induction of migration MCF-10A expressing CSF-1R and stimulated with CSF MCF-7 cDNA Completed - Data available Brugge
Migration Transwell assay for induction of migration MCF-10A expressing activated ErbB2 MCF-7 cDNA Completed - Data available Brugge
Migration Phagokinetic track assay (PKT) MCF-7 BC-1000 cDNA Completed - Data available Geiger (S.Naffar Abu-Amara), Brugge
Structure Localization REF52 GFP-tagged HT1080 cDNA Completed - Data available - Manabe et al., 2002 Horwitz
Structure Cytoskeletal localization HeLa with paxillin-YFP reporter siRNA-kinases Completed - Data available Geiger (S. Winograd-Katz), Brugge
Structure Morphological perturbations HeLa with paxillin-YFP reporter siRNA-phosphatases Completed - Data available Geiger (S. Winograd-Katz), Brugge
Structure Morphological perturbations HeLa with paxillin-YFP reporter siRNA-FA proteins Completed - Data available Geiger (A. Florentin), Brugge
Migration Phagokinetic track assay (PKT) MCF-7 MDA-MB-231 cDNA Completed - Data available Geiger (S.Naffar Abu-Amara), Brugge
Structure Cytoskeletal localization REF52 YFP-tagged rat cDNA Completed - Data available Geiger (I. Lavelin)
Invasive Cell translocation in response to metastasis signal PyMT and MTLn3   Completed - Data available Condeelis
Migration Transwell assay for induction of migration in the absence of a functional PI3K pathway MCF-10A stimulated with EGF + PI3K inhibition with LY294002 MDA-MB-231 cDNA Completed - no hits Brugge
Migration Phagokinetic track assay (PKT-B) and Wound healing assay MCF-10A siRNA-kinases Completed - Simpson et al, 2008 Data available / Geiger Brugge, Geiger
Migration Phagokinetic track assay (PKT-B) and Wound healing assay MCF-10A siRNA-phosphatases Completed - Simpson et al, 2008 Data available / Geiger Brugge, Geiger
Migration Phagokinetic track assay (PKT-B) and Wound healing assay MCF-10A siRNA-custom set Completed - Simpson et al, 2008 Data available / Geiger Brugge, Geiger
Structure Cytoskeletal localization REF52 YFP-tagged arabidopsis cDNA Nearing completion Sadot & Kam

Methods for validation of siRNA targeting sequences - Gary Borisy.

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Chemical Screens

Phenotype Screen Target Cell Library Status/Publication Lead laboratory (Collaborators)
Structure Morphological perturbations REF52 with YFP paxillin Tel Aviv HTS Natural Products Completed - Data available Geiger (Y. paran)
Structure Morphological perturbations REF52 with YFP paxillin Chem Bridge & Chemical Diversity Completed - Data available Geiger (Y. paran)
Structure Phagokinetic track assay (PKT) MCF-7 and MDA-231-MB Tel Aviv HTS Natural Products Initialized Geiger (S. Naffar Abu-Amara)
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