Gene Screens
Genes identified from the MCF-7 cDNA library that increase migratory behavior in MCF-10A cells as detected using the transwell migration assay.
For more details on the screen and PDEF, see the following: Gunawardane R. N., Sgroi D. C., Wrobel C. N., Koh E., Daley G. Q., and Brugge J.S. , A novel role for PDEF in epithelial cell migration and invasion. Cancer Res. 65(24):11572-80. (2005) PubMed.
If you have any questions related to this data please contact Dr. Joan Brugge
| Protein | Protein Class | Phenotype |
|---|---|---|
| RAN | small GTPase | ++ |
| SPDEF (PDEF) | transcription factor | ++ |
| DREV1 (CGI-81) | unknown | ++ |
| HM13 | aspartic protease | + |
| THUMPD2 (RP11) | unknown | + |
| BRD2 | protein kinase | + |
Phenotype Legend
| + | 2-> 3 fold over control | ++ | 3 --> 10 fold over control | +++ | 10-50 fold over control | ++++ | > 50 fold over control |
