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Biosensors - Resources

Publications - last updated August, 2008

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • El-Sibai M, Pertz O, Pang H, Yip SC, Lorenz M, Symons M, Condeelis JS, Hahn KM, Backer JM. RhoA/ROCK-mediated switching between Cdc42- and Rac1-dependent protrusion in MTLn3 carcinoma cells. Exp Cell Res 2008;314(7):1540-52.PubMed | CMC Update article.
  • Garrett SC, Hodgson L, Rybin A, Toutchkine A, Hahn KM, Lawrence DS, Bresnick AR. A Biosensor of S100A4 Metastasis Factor Activation: Inhibitor Screening and Cellular Activation Dynamics. Biochemistry 2008; 47 (3):986-96 PubMed | CMC Update article.
  • Hall B, McLean MA, Davis K, Casanova JE, Sligar SG, Schwartz MA. A fluorescence resonance energy transfer activation sensor for Arf6. Anal Biochem 2008; 374 (2.):243-9 PubMed | CMC Update article.
  • Healy KD, Hodgson L, Kim TY, Shutes A, Maddileti S, Juliano RL, Hahn KM, Harden TK, Bang YJ, Der CJ. DLC-1 suppresses non-small cell lung cancer growth and invasion by RhoGAP-dependent and independent mechanisms. Mol Carcinog 2008; 47 (5):326-37 PubMed | CMC Update article.
  • Hodgson L, Pertz O, Hahn KM. Design and optimization of genetically encoded fluorescent biosensors: GTPase biosensors. Methods Cell Biol 2008; 85:63-81 PubMed | CMC Update article.
  • Sabouri-Ghomi M, Wu Y, Hahn K, Danuser G. Visualizing and quantifying adhesive signals. Curr Opin Cell Biol 2008 Jun 27. [Epub ahead of print] PubMed | CMC Update article.
  • Shen F, Hodgson L, Price JH, Hahn KM. Digital differential interference contrast autofocus for high-resolution oil-immersion microscopy. Cytometry A 2008; 73(7):658-66. PubMed | CMC Update article.

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Birkenfeld J, Nalbant P, Bohl BP, Pertz O, Hahn KM, Bokoch GM. GEF-H1 Modulates Localized RhoA Activation during Cytokinesis under the Control of Mitotic Kinases. Dev Cell 2007; 12 (5):699-712 - PubMed | CMC Update article.
  • Cai X, Lietha D, Ceccarelli DF, Karginov AV, Rajfur Z, Jacobson K, Hahn KM, Eck MJ, Schaller MD. Spatial and Temporal Regulation of FAK Activity in Living Cells. Mol Cell Biol 2008 [Epub ahead of print] PubMed | CMC Update article.
  • El-Sibai M, Nalbant P, Pang H, Flinn RJ, Sarmiento C, Macaluso F, Cammer M, Condeelis JS, Hahn KM, Backer JM. Cdc42 is required for EGF-stimulated protrusion and motility in MTLn3 carcinoma cells. J Cell Sci 2007; 120 (Pt 19):3465-74 PubMed | CMC Update article.
  • Frantz C, Karydis A, Nalbant P, Hahn KM, Barber DL. Positive feedback between Cdc42 activity and H+ efflux by the Na-H exchanger NHE1 for polarity of migrating cells. J Cell Biol 2007; 179 (3):403-10 PubMed | CMC Update article.
  • Sainlos M, Imperiali B. Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization. Nat Protoc 2007; 2 (12):3201-9 PubMed | CMC Update article.
  • Sainlos M, Imperiali B. Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach. Nat Protoc 2007; 2 (12):3210-8 PubMed | CMC Update article.
  • Sainlos M, Imperiali B. Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN. Nat Protoc 2007; 2 (12):3219-25 PubMed | CMC Update article.
  • Toutchkine A, Han WG, Ullmann M, Liu T, Bashford D, Noodleman L, Hahn KM. Experimental and DFT Studies: Novel Structural Modifications Greatly Enhance the Solvent Sensitivity of Live Cell Imaging Dyes. J Phys Chem A 2007; 111 (42):10849-60 PubMed | CMC Update article.
  • Toutchkine A, Nguyen DV, Hahn KM. Simple One-Pot Preparation of Water-Soluble, Cysteine-Reactive Cyanine and Merocyanine Dyes for Biological Imaging. Bioconjug Chem 2007; 18 (4):1344-8PubMed | CMC Update article.
  • Toutchkine A, Nguyen DV, Hahn KM. Merocyanine Dyes with Improved Photostability. Org Lett 2007; 9 (15):2775-7 PubMed | CMC Update article.
  • Vogel EM, Imperiali B. Semisynthesis of unnatural amino acid mutants of paxillin: Protein probes for cell migration studies. Protein Sci 2007; 16 (3):550-6 PubMed | CMC Update article.

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Hodgson L, Perihan, Nalbant P, Shen F, Hahn K. Imaging and photobleach correction of MeroCBD, sensor of endogenous Cdc42 activation. Methods Enzymol. 2006; 406:140-56 PubMed | CMC Update article.
  • Moissoglu K, Schwartz MA. Integrin signalling in directed cell migration. Biol Cell 2006; 98 (9):547-55 PubMed | CMC Update article.
  • Moissoglu K, Slepchenko BM, Meller N, Horwitz AF, Schwartz MA. In vivo dynamics of Rac-membrane interactions. Mol Biol Cell. 2006; 17(6):2770-9 PubMed | CMC Update article.
  • Pertz O, Hodgson L, Klemke RL, Hahn KM. Spatiotemporal dynamics of RhoA activity in migrating cells. Nature, 2006; 440 (7087):1069-72 PubMed | CMC Update article.
  • Shen F, Hodgson L, Rabinovich A, Pertz O, Hahn KM, Price JH. Functional proteomics for cell migration. Cytometry A. 2006; 69 (7):563-72 PubMed
  • Shults MD, Carrico-Moniz D, Imperiali B. Optimal Sox-based fluorescent chemosensor design for serine/threonine protein kinases. Anal Biochem. 2006; 352(2):198-207 PubMed.

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Carrigan CN, Imperiali B. The engineering of membrane-permeable peptides. Anal Biochem. 2005; 341(2):290-8. PubMed
  • Rothman DM, Shults MD, Imperiali B. Chemical approaches for investigating phosphorylation in signal transduction networks. Trends Cell Biol. 2005; 15(9):502-10. PubMed.
  • Schults MD, Janes KA, Lauffenburger DA, Imperiali B. A multiplexed homogenous fluorescence-based assay for protein kinase activity in cell lysates. Nat Methods. 2005; 2(4):277-284. PubMed | CMC Update article
  • Vazquez ME, Blanco JB, Imperiali B. Photophysics and Biological Applications of the Environment-Sensitive Fluorophore 6-N,N-Dimethylamino-2,3-naphthalimide. J Am Chem Soc. 2005; 127(4):1300-6. PubMed.

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Nalbant P, Hodgson L, Kraynov V, Toutchkine A, Hahn KM. Activation of endogenous Cdc42 visualized in living cells. Science. 2004; 305(5690):1615-9. PubMed
  • Pertz O, Hahn KM. Designing biosensors for Rho family proteins--deciphering the dynamics of Rho family GTPase activation in living cells. J Cell Sci. 2004; 117(Pt 8):1313-8. PubMed
  • Tan X, Nalbant P, Toutchkine A, Hu D, Vorpagel ER, Hahn KM, Lu HP. Single Molecule Study of Protein-Protein Interaction Dynamics in a Cell Signaling System. J. Phys. Chem. B 2004; 108: 737-744.
  • Vazquez ME, Rothman DM, Imperiali B. A New Environment-Sensitive Fluorescent Amino Acid Building Block for Solid Phase Peptide Synthesis. Org. Biomol. Chem. 2004, 2:1965-1966.

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Shults MD, Imperiali B. Versatile fluorescence probes of protein kinase activity. J Am Chem Soc. 2003; 125(47):14248-9. PubMed
  • Toutchkine A, Kraynov V, Hahn K. Solvent-sensitive dyes to report protein conformational changes in living cells. J Am Chem Soc. 2003; 125(14):4132-45. PubMed
  • Tzima E, Kiosses WB, del Pozo MA, Schwartz MA. Localized cdc42 activation, detected using a novel assay, mediates microtubule organizing center positioning in endothelial cells in response to fluid shear stress. J Biol Chem. 2003; 278(33):31020-3. PubMed

2008 | 2007 | 2006 | 2005 | 2004 | 2003 | 2002

  • Del Pozo MA, Kiosses WB, Alderson NB, Meller N, Hahn KM, Schwartz MA. Integrins regulate GTP-Rac localized effector interactions through dissociation of Rho-GDI. Nat Cell Biol. 2002; 4(3):232-9. PubMed
  • Gardiner EM, Pestonjamasp N, Bohl BP, Chamberlain C, Hahn KM, Bokoch GM. Spatial and temporal analysis of Rac activation during live neutrophil chemotaxis: Roles in leading edge extension and tail retraction. Current Biology, 2002; 12:2029-2034. PubMed
  • Katsumi A, Milanini J, Kiosses WB, del Pozo MA, Kaunas R, Chien S, Hahn KM, Schwartz MA. Effects of cell tension on the small GTPase Rac.J Cell Biol. 2002; 158(1):153-64. PubMed
  • Nitz M, Mezo AR, Ali MH, Imperiali B. Enantioselective synthesis and application of the highly fluorescent and environment-sensitive amino acid 6-(2-dimethylaminonaphthoyl) alanine (DANA). Chem Commun (Camb) 2002; (17):1912-3. PubMed
  • Price JH, Goodacre A, Hahn K, Hodgson L, Hunter EA, Krajewski S, Murphy RF, Rabinovich A, Reed JC, Heynen S. Advances in molecular labeling, high throughput imaging and machine intelligence portend powerful functional cellular biochemistry tools. J Cell Biochem Suppl. 2002; 39:194-210. PubMed
  • Toutchkine A, Nalbant P, Hahn KM. Facile synthesis of thiol-reactive Cy3 and Cy5 derivatives with enhanced water solubility. Bioconjug Chem. 2002; 13(3):387-91. PubMed
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Protocols

Hahn lab biosensors (RhoA, Cdc42 and Rac) are available at addgene, a nonprofit respository of constructs (http://www.addgene.org/Klaus_Hahn). Additional information on these biosensors can be found at the Hahn lab biosensor page http://www.med.unc.edu/pharm/faculty/labpages/hahnlab/protocols.html

* Electronic supporting information files for articles appearing in ACS journals are available free of charge and without subscription via the Internet at http://pubs.acs.org. All files are copyrighted by the American Chemical Society but may be downloaded for personal use.

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