Biosensors - Protocols
Biosensor Initiative protocols describe techniques for developing novel probes and how they can be utilized to study signaling events in cell migration.
Software developed for analyzing data are described elsewhere.
Return to the Protocols index.
Synthesis of anhydride precursors of the environment-sensitive fluorophores 4-DMAP and 6-DMN.
Lab/Group: Department of Chemistry, Massachusetts Institute of Technology doi: 10.1038/nprot.2007.444 Publication date: December 2007
Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores through SPPS-based 'building block' approach
Lab/Group: Department of Chemistry, Massachusetts Institute of Technology doi: 10.1038/nprot.2007.443 Publication date: December 2007
Tools for investigating peptide-protein interactions: peptide incorporation of environment-sensitive fluorophores via on-resin derivatization
Lab/Group: Department of Chemistry, Massachusetts Institute of Technology doi: 10.1038/nprot.2007.442 Publication date: December 2007
Chelation-enhanced fluorophore chemistry
Lab/Group: Imperiali Laboratory Publication date: -
Environment-Sensitive fluorophore chemistry
Lab/Group: Imperiali Laboratory Publication date: -
Generation of a Dye-based Cdc42 (MeroCBD) Biosensor - expression, purification & labeling
Lab/Group: Hahn Laboratory Publication date: -
Generation of a Rac Biosensor - PBD expression, purification & labeling
Lab/Group: Hahn Laboratory Publication date: -
Generation of a Rho Single chain FRET Biosensor - sequence info & helpful hints
Lab/Group: Hahn Laboratory Publication date: -
Utilization of a Cdc42 Biosensor - Live Cell Activation Assay
Lab/Group: Schwartz Laboratory Publication date: -
